FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008021
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGAGGTTGTCCTGGTTTCTGCTGATACCAACTTAAGTAGTTCTTATGGTCGAAGTTGTACAAAACAAT64020.6351058162478758No Hit
CTCCTAGGCTGCTCATTTCCTGGGCATCTACCCGGAAACCCGGGGTCCCT59390.5891742334733105No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48740.4835216726635655No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41830.4149715134902944No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35880.3559449654322678No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33700.3343184318580664No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC30190.2994977287179533No Hit
GTTTATTACTGTCAGCAATATTTTAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGAA29620.2938430846182768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28790.28560912917488823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27650.2742998409755352No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTA26240.2603120371500197No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC25960.2575343172414067No Hit
GACTTCACTCTCACCATCAGCGGCCTGCAGGCTGACGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTATC25040.2484075232559639No Hit
GTCAGGGACCCCGGGTTTCCGGGTAGATGCCCAGGAAATGAGCAGCCTAGGAGGTTGTCCTGGTTTCTGCTGATA24640.24443935195794533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24340.24146322348443136No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24290.24096720207217903No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24280.24086799778972856No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23290.23104677382713257No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23010.22826905391851957No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22370.2219199798416898No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22260.2208287327347347No Hit
GTTTTGTACAACTTCGACCATAAGAACTACTTAAGTTGGTATCAGCAGAA22060.2188446470857254No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTACAACTTCGA22010.21834862567347307No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC21800.21626533574201331No Hit
CCTCCTAGGCTGCTCATTTCCTGGGCATCTACCCGGAAACCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGT21230.2106106916423368No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21000.2083289931459761No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20920.20753535888637242No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20680.20515445610756125No Hit
GTAATAAACTGCCACATCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA20240.20078946767974082No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19960.19801174777112776No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19500.1934483507784064No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGGTTTCCGGGT18180.18035338549494503No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17730.17588919278467413No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTACAACTTCGACCATAAGAACTA17640.17499635424261994No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGGCTGACGATG17280.1714250000744032No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17050.16914330157804253No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16870.16735762449393415No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16760.16626637738697905No Hit
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTATTCATTTGGAGAGACA16600.1646791088677716No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16510.1637862703257174No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15920.15793321766114No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15510.15386584208067094No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG15380.1525761864088149No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15320.1519809607141121No Hit
ACTTAAGTTGGTATCAGCAGAAACCAGGACAACCTCCTAGGCTGCTCATT15020.14900483224059816No Hit
CACCATCAGCGGCCTGCAGGCTGACGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTATCCCGTGGACGTT14980.1486080151107963No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14900.14781438085119258No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14270.1415645110568133No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14250.14136610249191237No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCC14090.13977883397270494No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13980.13868758686574983No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13820.13710031834654238No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13410.13303294276607333No Hit
CCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGAACTGTGG13400.13293373848362286No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13380.13273532991872194No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13270.13164408281176682No Hit
ATATTTTAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT13080.129759201445208No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13050.1294615885978566No Hit
GCTCATTTCCTGGGCATCTACCCGGAAACCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGACTT12890.12787432007864916No Hit
GTACAACTTCGACCATAAGAACTACTTAAGTTGGTATCAGCAGAAACCAGGACAACCTCCTAGGCTGCTCATTTC12850.1274775029488473No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12840.12737829866639683No Hit
CAGTAATAAACTGCCACATCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA12780.12678307297169406No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12680.1257910301471894No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12450.12350933165082871No Hit
AACCAGGACAACCTCCTAGGCTGCTCATTTCCTGGGCATCTACCCGGAAA12430.12331092308592777No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12390.12291410595612591No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12130.12033479461241382No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12020.11924354750545872No Hit
GTCCACGGGATACTAAAATATTGCTGACAGTAATAAACTGCCACATCGTC11860.11765627898625128No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11760.11666423616174663No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11750.11656503187929618No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11710.11616821474949432No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11680.11587060190214292No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11520.11428333338293548No Hit
TTATTACTGTCAGCAATATTTTAGTATCCCGTGGACGTTCGGCCAGGGGA11460.11368810768823269No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11400.11309288199352989No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11380.11289447342862897No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11240.11150561347432245No Hit
ACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTACAACTTCGACCATAA11210.11120800062697106No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11200.1111087963445206No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11200.1111087963445206No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11110.1102159578024664No Hit
CCACTGAAGCGGTCAGGGACCCCGGGTTTCCGGGTAGATGCCCAGGAAAT11030.1094223235428627No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10840.10753744217630386No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10780.10694221648160109No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC10560.10475972226769086No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10520.104362905137889No Hit
TTAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGA10520.104362905137889No Hit
GTGTATTACTGTGCGAGAGATCGACACAGTGACTTTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCC10510.10426370085543851No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA10510.10426370085543851No Hit
CTAAAATATTGCTGACAGTAATAAACTGCCACATCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTC10290.10208120664152831No Hit
GCCACAGTTCGGTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCACGG10270.10188279807662737No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10260.10178359379417691No Hit
GGAAATGAGCAGCCTAGGAGGTTGTCCTGGTTTCTGCTGATACCAACTTAAGTAGTTCTTATGGTCGAAGTTGTA10200.10118836809947411No Hit
GGTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGGCTGACGATGTGGCAGTTTATTACTGTCAGCAAT10180.10098995953457318No Hit
GAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCG10100.10019632527496947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)