FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008021
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTCCTGGGCATCTACCCGGAAACCCGGGGTCCCT51970.5155646558950656No Hit
GCCTAGGAGGTTGTCCTGGTTTCTGCTGATACCAACTTAAGTAGTTCTTATGGTCGAAGTTGTACAAAACAAT50740.5033625291536584No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47360.46983148168540134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36560.3626908566388994No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGGTTTCCGGGT33640.33372320616336365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30890.3064420284894858No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30640.3039619214282242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30030.2979104601987459No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29260.29027173045006005No Hit
GTTTATTACTGTCAGCAATATTTTAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGAA27950.27727596944904914No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27480.2726133681738773No Hit
GTAATAAACTGCCACATCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA27100.2688436054407597No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC26850.2663634983794981No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC24650.2445385562403958No Hit
GACTTCACTCTCACCATCAGCGGCCTGCAGGCTGACGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTATC24190.23997515924767443No Hit
GAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCG23020.22836825820097004No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22990.22807064535361865No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22830.2264833768344112No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22350.22172157127678888No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22330.22152316271188796No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTA21480.21309079870359843No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21170.210015465947634No Hit
GTCAGGGACCCCGGGTTTCCGGGTAGATGCCCAGGAAATGAGCAGCCTAGGAGGTTGTCCTGGTTTCTGCTGATA21140.20971785310028263No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20840.2067417246267687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20170.20009503770258755No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19500.1934483507784064No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19050.18898415806813548No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18350.18203985829660294No Hit
GTTTTGTACAACTTCGACCATAAGAACTACTTAAGTTGGTATCAGCAGAA18120.17975815980024226No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTACAACTTCGA18100.17955975123534132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18050.179063729823089No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17930.17787327843368342No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17740.1759883970671246No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGGCTGACGATG17440.17301226859361066No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17030.16894489301314158No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC16880.16745682877638463No Hit
CCTCCTAGGCTGCTCATTTCCTGGGCATCTACCCGGAAACCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGT16710.16577035597472672No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15530.1540642506455719No Hit
CAGTAATAAACTGCCACATCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA15330.15208016499656257No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15320.1519809607141121No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15260.1513857350194093No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14900.14781438085119258No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGATTGTTTTGTACAACTTCGACCATAAGAACTA14120.14007644682005632No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14070.13958042540780402No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13990.13878679114820028No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13980.13868758686574983No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13870.1375963397587947No Hit
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTATTCATTTGGAGAGACA13740.13630668408693866No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13530.1342233941554789No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13370.13263612563627147No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13350.13243771707137053No Hit
CACCATCAGCGGCCTGCAGGCTGACGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTATCCCGTGGACGTT13280.1317432870942173No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13230.13124726568196496No Hit
GTCCACGGGATACTAAAATATTGCTGACAGTAATAAACTGCCACATCGTC13210.13104885711706404No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13200.13094965283461354No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12990.1288663629031538No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12600.12499739588758567No Hit
ATATTTTAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT12590.12489819160513521No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12100.12003718176506242No Hit
CCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAACCGAACTGTGG11960.11864832181075592No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCACGGGATA11870.11775548326870174No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11610.11517617192498966No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11580.11487855907763828No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11530.11438253766538593No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11510.11418412910048502No Hit
CTAAAATATTGCTGACAGTAATAAACTGCCACATCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTC11430.11339049484088129No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11370.11279526914617849No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11230.11140640919187199No Hit
GGTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGGCTGACGATGTGGCAGTTTATTACTGTCAGCAAT11130.11041436636736734No Hit
TTATTACTGTCAGCAATATTTTAGTATCCCGTGGACGTTCGGCCAGGGGA11040.10952152782531316No Hit
GCTCATTTCCTGGGCATCTACCCGGAAACCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGACTT10940.1085294850008085No Hit
ACTTAAGTTGGTATCAGCAGAAACCAGGACAACCTCCTAGGCTGCTCATT10860.1077358507412048No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10750.10664460363424969No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10750.10664460363424969No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10670.10585096937464596No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10660.1057517650921955No Hit
TCGTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACC10630.1054541522448441No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCC10570.10485892655014131No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10550.1046605179852404No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10540.10456131370278991No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10540.10456131370278991No Hit
GGCTGACGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTATCCCGT10500.10416449657298806No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10480.10396608800808714No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA10310.10227961520642923No Hit
AACCAGGACAACCTCCTAGGCTGCTCATTTCCTGGGCATCTACCCGGAAA10280.10198200235907783No Hit
GTACAACTTCGACCATAAGAACTACTTAAGTTGGTATCAGCAGAAACCAGGACAACCTCCTAGGCTGCTCATTTC10150.10069234668722181No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10110.10029552955741992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)