FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820920
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21250.2588559177508162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17870.21768260000974518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16360.19928860303074597No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15390.1874725917263558No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15380.187350777176826No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14110.17188032938654194No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13330.16237879452321785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.15129367051600642No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12240.14910100862447012No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11950.14556838668810604No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11950.14556838668810604No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10880.13253422988841787No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10310.1255908005652195No Hit
GTGTGTACCTTGCATGCAGAAATAAATTCCAACATCCTCAGCCTCCACCC9980.12157092043073624No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9480.11548019295424646No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8910.1085367636310481No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8870.1080495054329289No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8710.10610047264045218No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8670.10561321444233299No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8500.10354236710032647No Hit
GGTACACACTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG8330.10147151975831994No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8260.10061881791161136No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8210.10000974516396238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.043.0988271
GTATCAA46650.039.669951
CTACGCT7800.037.7020128
ACTACGC7900.037.18834327
CTTATAC8300.036.8904461
TATACAC8450.036.6337783
TACGCTG8050.036.57367329
GTGGTAT4300.035.994821
TCTCCGA8350.035.85051711
ATCTCCG8350.035.44770410
TTATACA8750.034.9932252
ATCTCGT2950.034.87855537
CACGAGA8400.034.87026621
TGGTATC4650.034.732732
ACGCTGC8500.034.28761730
CGAGCCC8850.034.20720315
CCCACGA8600.034.03857819
CGCTGCA8700.033.9305731
CCGTCTT2800.033.8437747
TACACAT9150.033.4634675
Produced by FastQC (version 0.11.5)