FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820920
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75590.9207961798957267No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17950.2186571164059835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15290.18625444623105783No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14070.17139307118842273No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12430.15141548506553623No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12090.14727379038152316No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11830.14410661209374848No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11800.14374116844515908No Hit
GTGTGTACCTTGCATGCAGAAATAAATTCCAACATCCTCAGCCTCCACCC11770.14337572479656968No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10850.1321687862398285No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10430.12705257515957707No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9440.11499293475612728No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9230.11243482921600156No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9010.10975490912634606No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8870.1080495054329289No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8830.10756224723480973No Hit
GGTACACACTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG8660.10549139989280319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.039.8853871
GTATCAA45700.039.191761
TGGTATC5250.037.731642
GTGGTAT5900.034.742541
TATCAAC55050.032.3854682
ATCAACG57150.031.0684833
AACGCAG57250.031.0614876
TCAACGC57200.030.9732074
CAACGCA58400.030.4498315
CGCAGAG59850.029.9924188
ACGCAGA60300.029.6016677
AGAGTAC60750.029.4340411
CAGAGTA61550.029.16225810
GCAGAGT61800.028.9374739
ATCAGTG6000.027.71639370
CCCCGAT2254.3588443E-827.71639370
CCGATGT1007.5068994E-526.8091569
CGACTAT658.699045E-426.51528239
TGCGGGT1050.0096060926.39656370
AGCGGCT3200.024.38662365
Produced by FastQC (version 0.11.5)