FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136196
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49970.43980087942573287No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22520.19820523923689223No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22390.19706107044911267No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21850.19230836933064366No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21390.18825977208157746No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17730.15604702005639873No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17220.15155835788895577No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17030.1498861111991241No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16710.14706969572151285No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT16690.14689366975416213No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16000.14082077388056286No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15740.13853243630500373No Hit
GTGTGGTGGTCTCCACTCCCGCCCTGACGGGGCTGCTATCTGCCTTCCAG15370.1352759559090157No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15240.13413178712123613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15090.13281159236610585No Hit
TCTCTGGCCTGAGGACTGAGGACGAGGCTGACTATTACTGTCAGTCTTAT13960.1228661252107911No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCTCCCCATGATTGTCGCTATCATAAGA13580.11952163183112774No Hit
GTCTTATGATAGCGACAATCATGGGGAGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13130.1155610475657369No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGG12530.11028026854521579No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12210.10746385306760453No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12160.10702378814922778No Hit
GGGCCACACTGGTGTGTCTCATAAGTGGCTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11860.10438339863896723No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGTCAG11460.10086287929195316No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11430.1005988403409271No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11420.10051082735725175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG400.00687880352.63704370
GGTATCA22250.042.2469251
TGGTATC6150.041.62042
GTGGTAT6250.040.9689141
GTATCAA59100.039.5063251
TATCAAC70250.033.224212
ATCAACG72700.032.1745223
TCAACGC72900.032.02884
CAACGCA73750.031.6568685
AACGCAG74550.031.4962736
CCCCGAT950.005538854329.5506270
ACGCAGA79450.029.5114337
CGCAGAG80050.029.2482098
AGAGTAC82050.028.65827211
CAGAGTA82350.028.43132210
CGAATGC500.007467028727.79996543
GCTTACC500.00868341426.9532341
CTCGCTA500.0087095826.93661123
GACGTGT1859.094947E-1225.71295262
GCAGAGT91850.025.454069
Produced by FastQC (version 0.11.5)