FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047069
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32890.31411492461337315No Hit
GTATGGTAGTTCACCTCGAACGTTCGGCCCAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT30150.28794663961973854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27840.2658850562856889No Hit
CATCTATAGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27260.26034578427973704No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25080.23952576191253871No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22570.21555408478333327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20350.1943520436571038No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTAGTTGTCACTGACACTCTGA20340.194256538967346No Hit
GTGTATTCCTGTCAACAGTATGGTAGTTCACCTCGAACGTTCGGCCCAGGGACCAAGGTGGAAATCAGACGAA20080.19177341703364342No Hit
CTACCATACTGTTGACAGGAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC20050.19148690296437007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20030.1912958935848545No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18850.1800263401934352No Hit
GGAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18470.17639716198263916No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT17770.1697118336995938No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17340.16560513204000882No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17060.16293100072679068No Hit
GTATTCCTGTCAACAGTATGGTAGTTCACCTCGAACGTTCGGCCCAGGGA16410.1567231958925343No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGTGACAACTACTTAGCCTGGTACC16390.15653218651301873No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16160.15433557864858954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15860.15147043795585582No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15750.15041988636852013No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15360.1466952034679663No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15210.14526263312159943No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15040.143639053395717No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14930.1425885018083813No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14900.14230198773910793No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14680.14020088456443655No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTGA14570.13915033297710083No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13890.13265601407357108No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCC13690.13074592027841528No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13630.13017289213986852No Hit
ATAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGATACA13600.12988637807059517No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13570.12959986400132179No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTCCTGTCAACAGTATGGTAGTTCA13310.12711674206761925No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTGGGCCGAACGTT13200.12606619048028353No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13020.1243471060646433No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12480.11918985281772262No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12420.11861682467917588No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12370.11813930123038693No Hit
CTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGCATCCAGCAGGGCCA12340.11785278716111354No Hit
CAATAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGATA11890.11355507612201296No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11890.11355507612201296No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11860.1132685620527396No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11740.11212250577564611No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11690.11164498232685716No Hit
ACCATACTGTTGACAGGAATACACTGCAAAATCCTCAGGCTCCAGTCTGC11020.1052461681130852No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11020.1052461681130852No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10980.10486414935405404No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10920.1042911212155073No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10870.10381359776671832No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10840.10352708369744497No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10840.10352708369744497No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10720.10238102742035148No Hit
GCCCAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTC10720.10238102742035148No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10680.1019990086613203No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10630.10152148521253136No Hit
CCTCATCTATAGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10540.10066194300471125No Hit
GTATTATTGTGCGAAAGAGGGACATCCCGGGGGTTCGGGGAGTTCTCCCTCTGACTCCTGGGGCCAGGGAACC10490.10018441955592229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG250.006743742652.90397669
GGTATCA9250.042.724561
CGTATGC3050.038.4936741
GTATCAA22250.037.514351
TCGTATG3200.036.65023840
ATGCCGT3250.035.1935644
GTATGCC3500.034.5813242
ACGAGAC3350.034.5743522
ATCTCGT2500.034.3613737
GCCGTCT3400.032.71529846
CTTATAC3550.032.6296121
TATCAAC25500.032.329212
CACGAGA3600.032.1733521
TCTTATA1700.032.0620232
CCCACGA3750.031.78876719
ATCTCCG3750.031.78269410
ACCGAGG3550.031.70324327
TACACAT3900.031.4439455
GAGACCG3700.031.31726624
GACCGAG3600.031.24946826
Produced by FastQC (version 0.11.5)