FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047069
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30810.2942499491437527No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30680.2930083881769014No Hit
GTATGGTAGTTCACCTCGAACGTTCGGCCCAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT27430.2619693640056195No Hit
CATCTATAGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23640.22577308658741688No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21690.2071496720846477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21290.2033294844943361No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20950.2000823250425712No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20560.19635764214201737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19280.1841330418530202No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTAGTTGTCACTGACACTCTGA18110.17295899315135868No Hit
GTGTATTCCTGTCAACAGTATGGTAGTTCACCTCGAACGTTCGGCCCAGGGACCAAGGTGGAAATCAGACGAA18040.17229046032305417No Hit
CTACCATACTGTTGACAGGAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC17890.1708578899766873No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT17730.16932981494056265No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17280.16503210390146208No Hit
GGAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16070.1534760364407694No Hit
GTATTCCTGTCAACAGTATGGTAGTTCACCTCGAACGTTCGGCCCAGGGA14860.14191996898007675No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGTGACAACTACTTAGCCTGGTACC14790.14125143615177224No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14450.13800427670000737No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14090.1345661078687269No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14050.13418408910969573No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13940.13313353752236004No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13890.13265601407357108No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13880.13256050938381328No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13840.13217849062478212No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTGA13650.1303639015193841No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13560.129504359311564No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13520.12912234055253283No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13370.12768977020616598No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTGGGCCGAACGTT13060.12472912482367447No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12770.12195948882069854No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12760.12186398413094074No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12550.11985838564602715No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTCCTGTCAACAGTATGGTAGTTCA12550.11985838564602715No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12370.11813930123038693No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCC12310.11756627309184019No Hit
ATAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGATACA12270.11718425433280902No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11840.11307755267322402No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11660.11135846825758378No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11590.11068993542927925No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11430.10916186039315459No Hit
CTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATAGTGCATCCAGCAGGGCCA11180.10677424314920983No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11080.10581919625163194No Hit
ACCATACTGTTGACAGGAATACACTGCAAAATCCTCAGGCTCCAGTCTGC10870.10381359776671832No Hit
CAATAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGATA10780.10295405555889822No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10760.10276304617938263No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10760.10276304617938263No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10620.10142598052277356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)