FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454882
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57380.3943962465684502No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47410.32586835221000743No Hit
GTGTGTACCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACCC46090.3167954514524202No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA42450.29177624027240695No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40410.27775448455613583No Hit
GTACACACTGGCCTCCGAGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38440.2642138675164034No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36300.24950477083364836No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA36260.2492298344470548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34370.23623909018050948No Hit
GGCCAAAACTGCTCGGAGGCCAGTGTGTACCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACCCTG34360.23617035608386108No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31660.21761214998879636No Hit
GGTACACACTGGCCTCCGAGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30720.21115114490384787No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30390.20888291971445108No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC29670.20393406475576714No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29420.20221571233955743No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29370.2018720418563155No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27810.19114952277916697No Hit
GTCTGGGACACCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA27360.1880564884299895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27210.1870254769802637No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT27190.1868880087869669No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27140.18654433830372497No Hit
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATACA27070.18606319962718626No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26810.18427611311432815No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC26780.184069910824383No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26610.1829014311813604No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26180.17994586502547974No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA25760.17705903296624742No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA25660.17637169199976355No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG25480.17513447826009257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC25080.17238511439415705No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAG24840.17073549607459573No Hit
AGGTACACACTGGCCTCCGAGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22610.1554077925220052No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG22390.1538956423957407No Hit
CTCCCTGCCCGTCGCCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA22300.1532770355259052No Hit
GTCTCCACTCTCCCTGCCCGTCGCCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA22280.15313956733260842No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGCGACGGGCAGGGAGAGTGGAGA22240.15286463094601488No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCC21830.15004653298343096No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT20920.14379173018842767No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20690.14221084596551473No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20520.14104236632249215No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20000.13746819329677598No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19820.13623097955710498No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19790.1360247772671598No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19450.13368781798111462No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC19440.13361908388446622No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19300.13265680653138878No Hit
GTCCAAGGGCGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC19260.13238187014479524No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19240.13224440195149847No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTC19230.1321756678548501No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT19160.13169452917831137No Hit
AGTGTGTACCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACC19160.13169452917831137No Hit
GGACTCTGGTGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGCACTGATT19150.13162579508166297No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18790.12915136760232102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18250.12543972638330805No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18160.12482111951347258No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17760.12207175564753704No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC17700.12165935106764672No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17480.12014720094138218No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17430.11980353045814025No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17290.11884125310506281No Hit
GTATATTACTGTGCGAAAGATCGGGGGTCGATGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCC16830.11567948465923696No Hit
GTTTCTAACCGGGACTCTGGTGTCCCAGACAGATTCAGTGGCAGTGGGTC16750.11512961188604987No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC16650.11444227091956599No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16590.11402986633967567No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16580.11396113224302726No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16480.1132737912765434No Hit
GTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTT16290.11196784344022401No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16140.1109368319904982No Hit
CCACTCTCCCTGCCCGTCGCCCTTGGACAGCCGGCCTCCATCTCCTGCAG16120.11079936379720143No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16090.11059316150725626No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15970.1097683523474756No Hit
GTAATAAATTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA15680.10777506354467237No Hit
CCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAACCAA15530.10674405209494653No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGGTACACACTGG15480.10640038161170459No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14910.10248253810274649No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14830.10193266532955937No Hit
CCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCC14690.10097038797648195No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14620.10048924929994324No Hit
GGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGG14570.10014557881670129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGTCG200.007675711551.2021145
GTGGTAT3650.042.086821
GGTATCA13400.041.270211
GTCGTAT350.001349777439.41759539
TCGTATG3800.039.0652440
CGTATGC4000.038.87460741
TGGTATC4250.037.749012
ATGCCGT3950.037.74181744
GTATCAA35500.037.2142331
GACAGGT3500.037.0631181
CCCACGA4150.036.1972719
CGAGACG4250.036.1575823
TATACAC4750.035.212683
TCTTATA1950.035.009992
ACGAGAC4300.034.9429822
CACGAGA4300.034.9381821
GCTACAT4150.034.69267732
GTATGCC4300.034.59613442
ATCTCGT3900.034.41986537
CTTATAC4900.034.1370851
Produced by FastQC (version 0.11.5)