FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856758
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51380.27671888312854986No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43310.23325603013424476No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42710.2300245912499098No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38010.2047116533226193No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37610.20255736073306269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT36470.19641762685282627No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36400.19604062564965385No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35030.1886621735304224No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34540.18602316510821548No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCACCCTTTGGGTCTTCGG34090.18359958594496428No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT33030.1778907105826392No Hit
CCCTTTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC30710.16539581356321073No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30350.16345695023260973No Hit
GTAGTATATAGCTGTGTCCCCGGCGCTCAGGCGGTTCATTTGCAGATAGA29640.15963308088614672No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAAAGGGTGGTG29460.15866364922084625No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG29320.1579096468145014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29270.15764036024080683No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28210.15193148487848174No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28150.15160834099004825No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACATT27710.14923861914153597No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27450.14783832895832413No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27230.14665346803406798No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACATTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTA25870.1393288732295754No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAAAGGGTGGTGC25080.13507414536520107No Hit
TCTCTGGGCTCCAGGTTGAGGACGAGGCTGATTATTACTGCAGCTCATAT24870.13394314175568384No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24670.1328659954609055No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAAAGGGTGGTGCTGCTTGTATATGAG24340.13108870407452128No Hit
GTAATAATCAGCCTCGTCCTCAACCTGGAGCCCAGAGATGGTCAGGGAGG24300.13087327481556563No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG24000.12925755537339814No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC23760.12796497981966418No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23180.12484125556480705No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22880.12322553612263956No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22320.12020952649726027No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22260.11988638260882678No Hit
CCCAAAGGGTGGTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCG22090.11897080825826521No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22080.11891695094352629No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21970.11832452048139823No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21420.11536236817075785No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCG21360.11503922428232435No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21010.1131542182664623No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20650.11121535493586134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6400.061.46538570
ATCTCGT7750.041.43758837
TCGTATG9150.041.38715440
CGTATGC8900.041.1021141
TCTCGTA7850.041.00839238
CTTATAC9800.040.866911
TATACAC9900.040.794063
TTATACA9900.040.1141622
GTATCAA49900.038.713481
GGTATCA18750.038.5911871
ACGAGAC10050.038.51622
ATGCCGT9300.038.48036244
CATCTCG8200.038.2654436
CACGAGA10250.038.08977521
GTGGTAT5850.037.969861
TACACAT10800.037.3945585
GTATGCC9850.037.2170942
TACATCT8800.037.0862534
GCCGTCT9600.036.7122446
TCTACAT9500.036.42140232
Produced by FastQC (version 0.11.5)