FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856758
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87360.4704975015591693No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC47480.2557145303803727No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45300.243973635767289No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37560.2022880741593681No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36840.19841034749816616No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35660.19205518435897406No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34780.18731574066194948No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32890.1771367081762944No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCACCCTTTGGGTCTTCGG31440.16932739753915158No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31120.16760396346750628No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG29820.1606025125514472No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29260.1575865029260679No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29050.15645549931655067No Hit
CCCTTTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC28820.15521678107755563No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27890.15020805080683644No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27880.15015419349209752No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27440.1477844716435852No Hit
GTAGTATATAGCTGTGTCCCCGGCGCTCAGGCGGTTCATTTGCAGATAGA27090.14589946562772316No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACATT26750.14406831692660002No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25650.13814401230531928No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25300.13625900628945722No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25090.13512800267994No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAAAGGGTGGTG23970.12909598342918138No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAAAGGGTGGTGCTGCTTGTATATGAG23690.12758797861649177No Hit
TCTCTGGGCTCCAGGTTGAGGACGAGGCTGATTATTACTGCAGCTCATAT23400.1260261164890632No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23230.12511054213850162No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACATTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTA23210.1250028275090238No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC23120.12451811167637356No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22620.12182524593942776No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22360.12042495575621594No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22320.12020952649726027No Hit
GTAATAATCAGCCTCGTCCTCAACCTGGAGCCCAGAGATGGTCAGGGAGG21600.11633179983605833No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20960.11288493169276771No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAAAGGGTGGTGC20700.1114846415095559No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCG20580.11083835373268891No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20430.11003049401160517No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20390.10981506475264952No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20360.10965349280843276No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20300.10933034891999927No Hit
CCCAAAGGGTGGTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCG19950.10744534290413721No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19540.10523719299984166No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19280.10383690281662984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8900.039.341671
TGGTATC9500.036.49272
GTATCAA52450.036.3954431
GGTATCA21850.033.5903471
TATCAAC60100.031.9221972
AACGCAG63400.030.3537226
ATCAACG62900.030.2773443
TCAACGC63650.029.8644964
CAACGCA64550.029.656595
AATGGTA4950.029.40549970
AGGATGG12800.028.73553369
CGCAGAG67750.028.6531058
ACGCAGA67400.028.6022327
GACAGGT3700.028.2078131
GTCATAG11050.028.0307671
AGAGTAC69100.027.99141111
TCAGGTC10650.027.7988312
GCAGAGT69950.027.7030939
CAGAGTA70000.027.63226710
TAGGTGG10950.027.34525
Produced by FastQC (version 0.11.5)