FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782085
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGGCAGCCTGAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG172610.6204339551092077No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG164540.5914269333970745No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGTCAGGGAGG158250.5688179908234292No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC153430.5514928551787598No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTTCAGGCTGCCATCCCATGCTGCACAGTAATAATCAG152480.5480781500205781No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTG145070.5214434497867606No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTT136520.4907111033631251No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG128680.46253079974192013No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT126860.45598894354414043No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC119070.4279883612470503No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC112720.4051637530844672No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC97840.3516786870278945No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT95070.3417221256719331No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG89510.3217371144303643No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG88420.31781918956466104No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT83290.29937978171047974No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT75240.2704446485279925No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTTCAGGCTGCCATCCCATGCTGCACAGTAATAATCAGC75160.27015709440940877No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC73770.26516084159901654No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA70680.2540540637687202No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG70660.2539821752390743No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA70580.25369462112049057No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG70030.25171768655522747No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC68760.24715276492271085No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG68110.24481638770921807No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA67790.24366617123488318No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC66910.24050307593046222No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCAC64520.23191239663777347No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGACCATCAGTGGCCTCCGGT64250.2309419014875534No Hit
CCCCTGAGGGCCGCTGATTATTCTTATAAATGAGGAGTTTGGGGGCCGCTCCTGGGAACTGCTGGTACCAGTATA63760.22918063251122808No Hit
GCATGGGATGGCAGCCTGAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC63640.2287493013333525No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG61730.2218839467521661No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA61650.22159639263358236No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC61360.22055400895371635No Hit
CTCCTGGGAACTGCTGGTACCAGTATACAGATTTAGTTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGGTGA61140.21976323512761112No Hit
TCCCAGGAGCGGCCCCCAAACTCCTCATTTATAAGAATAATCAGCGGCCC60550.21764252350305616No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC59240.2129338248112477No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG58810.21138822142386018No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC57040.20502608655019525No Hit
TCTGTATACTGGTACCAGCAGTTCCCAGGAGCGGCCCCCAAACTCCTCAT55280.19869989594135332No Hit
CCTTCAGGCTGCCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCG53670.19291286930485588No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT53640.192805036510387No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA50300.1807996520595165No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT48900.17576745498430135No Hit
GTATACTGGTACCAGCAGTTCCCAGGAGCGGCCCCCAAACTCCTCATTTA47810.1718495301185981No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG47770.17170575305930624No Hit
GTATACAGATTTAGTTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGGTGACCCTCTGCCCGGGGGCCCCAGA47600.17109470055731582No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA46810.16825510363630156No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC45590.16386990332789975No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC45110.16214457861639742No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATG44810.16106625067170843No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT44680.1605989752290099No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT44430.15970036860843576No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG44060.15837043080998603No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC43830.15754371271905782No Hit
GCCTGAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC43720.1571483258060052No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC43620.15678888315777556No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA43160.15513544697591913No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC42760.15369767638300053No Hit
GTTTGGGGGCCGCTCCTGGGAACTGCTGGTACCAGTATACAGATTTAGTT42690.15344606652923976No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA42000.15096591225645514No Hit
CTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAAATCTGTATACTGGTACCAGCAGTTCCCAGGAGCGGCCCC41520.1492405875449528No Hit
GTGCAGCATGGGATGGCAGCCTGAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTG41100.14773092842238825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40800.14665260047769926No Hit
GTCTGGCACCTCAGCCTCCCTGACCATCAGTGGCCTCCGGTCCGAGGATG40710.1463291020942926No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA40330.14496322003101988No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC39800.14305817399540274No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC39230.1410093509004937No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGGCAGCCTGA39110.1405780197226181No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC37960.13644442926797706No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGG37860.1360849866197474No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG37710.13554582264740295No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC37650.13533015705846516No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC37530.13489882588058955No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC37070.13324538969873315No Hit
CCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGG36290.13044173704254183No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG35670.12821319262351796No Hit
GACCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGGCAGCCTGAAGGTGTT35550.12778186144564238No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGG35240.12666758923613045No Hit
GCCCCCAAACTCCTCATTTATAAGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT35180.12645192364719265No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC34670.12461876614122142No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG34660.12458282187639846No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGC34590.1243312120226377No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC33640.12091650686445597No Hit
TCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG33500.12041328715693446No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGAC33470.12030545436246555No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAAGCAG33210.11937090347706847No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA33090.11893957229919287No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC32810.11793313288414983No Hit
CCTGGGAACTGCTGGTACCAGTATACAGATTTAGTTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGGTGACC32750.11771746729521204No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT32750.11771746729521204No Hit
GTTCCCAGGAGCGGCCCCCAAACTCCTCATTTATAAGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCT32700.11753774597109722No Hit
CCAGTATACAGATTTAGTTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGA32480.11674697214499197No Hit
CTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC32300.1160999753781786No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT32060.11523731302242743No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG32050.11520136875760446No Hit
TCCTCGGACCGGAGGCCACTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC31530.11333226698681026No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG31260.11236177183659019No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG31100.11178666359942273No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC31050.1116069422753079No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC30870.11095994550849454No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG30810.11074427991955672No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA30780.11063644712508784No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG30730.11045672580097302No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC30610.11002539462309742No Hit
GGGTCACCATCTCTTGCTCTGGAAGCAGCTCCAACATCGGAACTAAATCT30450.10945028638592999No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT30340.10905489947287736No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC30060.10804846005783432No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG29990.10779685020407356No Hit
CCTTGGTCCCTCCGCCGAACACCTTCAGGCTGCCATCCCATGCTGCACAG29770.10700607637796833No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTCTTATAAATGAGGAGTTTGGGGGCCGCTCCTGGGAACTGCTGGTA29750.1069341878483224No Hit
GGATGGCAGCCTGAAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT29090.10456186637000665No Hit
GCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAG28820.10359137121978661No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG28780.10344759416049475No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG28310.10175821371381537No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT27880.10021261032642784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.047.051491
GTGGTAT5500.044.819671
TAACGGT350.00150591238.550664
GTATCAA54900.037.8893241
TGGTATC6150.037.8740582
GCCAGAG72450.034.82637470
TATCAAC61600.033.4285052
ATCAACG64200.032.2156453
TCAACGC64350.032.02824
CAACGCA65650.031.4453585
AACGCAG66150.031.3096626
ATGGCAA5250.030.12741370
ACGCAGA70350.029.3445327
CGCAGAG71300.029.0008568
AGAGTAC71450.028.93841411
TACGTAC2450.028.91403612
GAATACG2450.028.9129969
CCGAAAT854.0421346E-628.749147
CAGAGTA72450.028.5865710
AATGGTA6350.028.46684670
Produced by FastQC (version 0.11.5)