FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034996
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33160.32038771164333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30900.29855187846136605No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24640.23806855292194365No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24290.23468689734066606No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCAGCGCCGAACACTTCGAGAGTGTTGCTGCCTGTATATGAA21950.2120781143115529No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAAGTTGTAGACTCA20750.20048386660431539No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19580.1891794750897588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18660.18029055184754336No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18390.17768184611341495No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17650.17053206002728513No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17330.16744026063868847No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15910.15372040085179073No Hit
TCTCTGGGCTCCAGGCTGATGACGAGGCTGATTATTACTGTAGTTCATAT15710.1517880262339178No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15610.15082183892498136No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCAG15210.14695708968923551No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15080.14570104618761812No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15020.14512133380225625No Hit
GACCTAGGACGGTCAGCTTGGTCCCAGCGCCGAACACTTCGAGAGTGTTG14990.1448314776095753No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14740.14241600933723417No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14600.1410633471047231No Hit
GTATATTACTGTGCGAAGCTGCGGGCAGCTCGTCTTTCTGGTCCTGACTA14110.13632902929093446No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT13980.13507298578931706No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13950.13478312959663613No Hit
GATTATTACTGTAGTTCATATACAGGCAGCAACACTCTCGAAGTGTTCGG13640.1317879489389331No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13430.12975895559016654No Hit
CTCTCGAAGTGTTCGGCGCTGGGACCAAGCTGACCGTCCTAGGTCAGCCC12880.124444925391016No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12780.12347873808207954No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12710.12280240696582402No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12710.12280240696582402No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12690.12260916950403673No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATGACTATGTCTCTTGGTACCAACAACACCCAGGCAAAGC12550.12125650727152569No Hit
CTTCGAGAGTGTTGCTGCCTGTATATGAACTACAGTAATAATCAGCCTCG12540.12115988854063205No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12430.12009708250080194No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12370.11951737011544004No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12280.11864780153739724No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12100.11690866438131163No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11800.11401010245450224No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATGACTATGTCTCTTGGTACCAACAACA11680.11285067768377849No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11520.11130477798948016No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11290.10908254717892629No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11260.10879269098624535No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11260.10879269098624535No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11060.10686031636837244No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT10650.10289894840173296No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10600.10241585474726471No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10570.10212599855458379No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10430.10077333632207273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3100.054.6192051
TGGTATC3650.047.3006742
GTATCAA27550.039.8254281
GGTATCA10750.039.3766371
GACAGGT2400.033.8639071
CTTATGG400.00284895533.8524482
TATCAAC33150.032.9844362
TCTGGAG8300.032.60279570
ATCAACG33450.032.3787423
TCAACGC34300.031.7660264
CAACGCA34950.031.4626545
GTCACCG7050.031.121770
AACGCAG35950.030.6815916
CGGGATT450.00507250930.0896117
TTAGGTC450.005082109530.0779784
CGCAGAG37900.029.3708048
AGAGTAC38700.028.76087611
CAGAGTA38900.028.69997610
GCAGAGT39200.028.2241489
TAGTTCG657.1598723E-427.41479557
Produced by FastQC (version 0.11.5)