FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368648
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG80210.5860528053962742No Hit
GATTATTACTGTGCAGTATGGGATGACAGCCTCAATGGTCCGGTGTTCGG78460.5732664644232849No Hit
GTATGGGATGACAGCCTCAATGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG65700.48003577252880214No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50630.3699271105499734No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47630.3480076688819915No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTGAGGCTGTCATCCCATA46520.3398974754648383No Hit
ATCCCATACTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC45810.3347098742700826No Hit
GTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTG45340.3312758284087654No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41460.30292668385150895No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCT37920.27706174268329037No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36490.266613475488219No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35530.2595992541544649No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33490.2446940338202372No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG32160.23497641468076527No Hit
TTACTATAGATGAGGAATTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA32030.23402657220848605No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31180.22781606373589117No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31120.22737767490253158No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30580.22343217540229482No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTGAGG30310.22145942565217647No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29500.21554117640182135No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29410.2148835931517819No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29170.21313003781834333No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCAC28780.21028051040150572No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28580.20881921429030692No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28430.20772324220690785No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28200.20604275167902925No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC28140.2056043628456696No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27700.20238951140103223No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26850.1961790029284374No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26680.19493690123391844No Hit
GCTCATTACTATAGATGAGGAATTTGGGGGCCGTTCCTGGGAGCTGCTGG25610.1871189670390049No Hit
GTCTGTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTGCTCTGGAAGCTA24240.17710908867729322No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24160.1765245702328137No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24060.1757939221772143No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTGCTCTGG23140.16907196006569988No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23140.16907196006569988No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22530.16461500692654357No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22410.16373822925982429No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC22190.16213080353750564No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT22050.16110789625966646No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21840.15957353534290775No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21760.15898901689842823No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGTATGGGATGACAGCCTCAATGGTCCGGTGTTCGGCGGAGGGAC21460.15679707273163004No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGATC20920.15285157323139334No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20700.15124414750907464No Hit
GTGCAGTATGGGATGACAGCCTCAATGGTCCGGTGTTCGGCGGAGGGACC20510.1498559162034358No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20300.14832155528667706No Hit
GTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAAG19760.14437605578644033No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC19430.14196491720296234No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19150.13991910264728402No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC18590.13582747353592745No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18550.13553521431368767No Hit
CTATAGTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC18330.133927788591369No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18310.13378165898024913No Hit
CCATTGAGGCTGTCATCCCATACTGCACAGTAATAATCAGCCTCATCCTC18110.13232036286905033No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18100.1322472980634904No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17930.13100519636897143No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG17670.12910551142441298No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17570.1283748633688136No Hit
GTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17530.12808260414657385No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17510.12793647453545395No Hit
ATACTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG17280.12625598400757537No Hit
CATTACTATAGATGAGGAATTTGGGGGCCGTTCCTGGGAGCTGCTGGTAC17280.12625598400757537No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17070.12472162309081662No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG17070.12472162309081662No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17010.12428323425745699No Hit
GTCTCTGGTGGCTCCATCAGTAGTTCCCACTGGAGCTGGATCCGGCAGCC16710.1220912900906588No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16500.12055692917390008No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16290.11902256825714135No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16240.11865724422934167No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16160.11807272578486215No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16070.11741514253482269No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTGAGGC16050.11726901292370281No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15890.11609997603474378No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15810.11551545759026427No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15360.11222754134006699No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15150.11069318042330825No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14950.10923188431210948No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14850.10850123625651008No Hit
GCTTGGTCCCTCCGCCGAACACCGGACCATTGAGGCTGTCATCCCATACTGCACAGTAATAATCAGCCTCATCCT14650.10703994014531128No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14480.10579783845079231No Hit
ATAGTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG14480.10579783845079231No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14470.10572477364523238No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGATCCCTGAGGGCCGCTG14150.10338669986731432No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14120.1031675054506345No Hit
CCCAGGAACGGCCCCCAAATTCCTCATCTATAGTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGG14000.10229072778391522No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14000.10229072778391522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3700.047.1314281
AGGGGGG3450.046.7705470
CGCGTAT306.350014E-445.9437839
GGTATCA13150.043.424311
TGGTATC4100.043.3656462
CTACGCT4600.041.67728
TATACAC4800.041.3155023
CGTATGC1900.039.99427841
TTATACA5000.039.6628882
GTATCAA33200.039.5489851
ACTACGC4850.039.5099127
TACGCTG4850.038.8327829
TACACAT5400.038.621635
CTTATAC5150.038.5090641
GACTACG5100.038.23011826
CACGAGA5200.038.1373921
CCCACGA5150.037.84234619
GCCAGAG36950.037.78350470
ACGAGAC5250.037.77417822
ACACATC5250.037.771426
Produced by FastQC (version 0.11.5)