FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368648
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGTATGGGATGACAGCCTCAATGGTCCGGTGTTCGG70390.5143031663364137No Hit
GTATGGGATGACAGCCTCAATGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG61290.44781419327686883No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC49220.35962497296602197No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48360.35334139968786715No Hit
GTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTG47750.34888444654871087No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45770.33441761504784284No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCT40510.2959855273233147No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG39990.29218615743419785No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTGAGGCTGTCATCCCATA37320.272677854349694No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36810.26895154926613707No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32340.23629158118084417No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31690.23154236881944812No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31490.23008107270824932No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG31110.22730461009697162No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30250.2210210368188168No Hit
ATCCCATACTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC29940.21875602784645867No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29240.21364149145726297No Hit
TTACTATAGATGAGGAATTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA29110.21269164898498372No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28920.21130341767934485No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28340.2070656589568684No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCAC28180.20589662206790937No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26340.1924526978448805No Hit
GTCTGTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTGCTCTGGAAGCTA26160.1911375313448016No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26100.19069914251144193No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26000.18996849445584257No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25270.18463476364996698No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24820.1813468473997697No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTGAGG24500.17900877362185164No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24460.17871651439961186No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24390.17820506076069229No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATTTCTTGCTCTGG24100.17608618139945406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23890.17455182048269532No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC23760.17360197801041613No Hit
GCTCATTACTATAGATGAGGAATTTGGGGGCCGTTCCTGGGAGCTGCTGG23700.1731635891770565No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23170.1692911544823797No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23050.16841437681566043No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22580.16498033095434325No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22540.1646880717321035No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22480.16424968289874387No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGTATGGGATGACAGCCTCAATGGTCCGGTGTTCGGCGGAGGGAC21980.1605964426207469No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC21240.15518964700931137No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20920.15285157323139334No Hit
GTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20600.15051349945347525No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20140.14715251839771806No Hit
GTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAAG19970.14591041670319907No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19420.1418918523974024No Hit
CTATAGTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC19190.1402113618695238No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19180.14013829706396386No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18860.13780022328604577No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18800.13736183445268615No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18410.13451230703584852No Hit
GTGCAGTATGGGATGACAGCCTCAATGGTCCGGTGTTCGGCGGAGGGACC18350.13407391820248887No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG18340.13400085339692894No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC18220.13312407573020968No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18170.13275875170240997No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17610.12866712259105337No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17450.12749808570209434No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17300.12640211361869522No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16900.12347952139629766No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16650.12165290125729918No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16370.11960708670162086No Hit
ATAGTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG16330.11931482747938112No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG16240.11865724422934167No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15820.1155885223958242No Hit
GGGTCACCATTTCTTGCTCTGGAAGCTACTCCAACATCGGAAGTAATTCT15700.11471174472910492No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15570.11376190225682573No Hit
GTCTCTGGTGGCTCCATCAGTAGTTCCCACTGGAGCTGGATCCGGCAGCC15450.11288512459010644No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGATCCCTGAGGGCCGCTG15100.11032785639550856No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15030.109816402756589No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC15020.10974333795102904No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14910.10893962508986971No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14890.10879349547874982No Hit
CATTACTATAGATGAGGAATTTGGGGGCCGTTCCTGGGAGCTGCTGGTAC14720.10755139378423086No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14530.10616316247859202No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14060.10272911661727485No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14060.10272911661727485No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14030.10250992220059503No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13990.10221766297835529No Hit
CCCAGGAACGGCCCCCAAATTCCTCATCTATAGTAATGAGCAGCGGCCCTCAGGGATCCCTGACCGATTCTCTGG13890.10148701492275589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATCG150.00236300768.9456740
TTATCGA200.007333908751.7952841
GGTATCA13150.044.163021
GTGGTAT3500.043.9217951
ACCGTAC350.001010604941.80624867
AATCGGA350.001156390240.67669763
GTATCAA35400.040.1444631
TGGTATC4000.036.7127532
TATCAAC40000.035.688212
TACCATA400.002375448435.13155756
ATCAACG41000.035.0522773
TCAACGC41600.034.7006874
TTAATCC400.00273936434.1251959
CAACGCA42700.034.046525
AACGCAG43600.033.7350856
ACGCAGA45300.032.3937537
CGTACTA550.003649223832.18444469
GACCGTA450.003726368832.0477866
CGCAGAG46500.031.6311748
AGCGTAT450.00432300631.09005552
Produced by FastQC (version 0.11.5)