FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116975
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46160.4132590254929609No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39440.35309653304684524No Hit
GTACTACTGTCAACAGAATTACTACTACCCTCGCACTTTTGGCCAGGGGA34460.3085118288233846No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33980.3042145079343763No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30920.2768190872669487No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30620.2741332617113185No Hit
CATCTATGCTGCATCCACTTTACATGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA29520.26428523467400794No Hit
GGTAAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATAGGAACTA26310.2355469012287652No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25980.23259249311757205No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGG25730.23035430515454688No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGTTCCTATTTAAATTGGTATCAGCAGAAA25700.23008572259898388No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25640.22954855748785782No Hit
GAATTACTACTACCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT25420.22757895208039572No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24920.22310257615434542No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24740.22149108082096733No Hit
GCATAGATGAGGAGGTAAGGGGCTTTCCCGGGTTTCTGCTGATACCAATT24570.21996911300611027No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24120.215940374672665No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24050.21531368204301798No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23260.20824100807985854No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23090.20671904026500146No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22340.20000447637592605No Hit
GGCCAAAAGTGCGAGGGTAGTAGTAATTCTGTTGACAGTAGTACGTTGCA22160.19839298104254796No Hit
CAGTAGTACGTTGCAAAATCTTCAACCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA21980.19678148570916984No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA21910.19615479307952283No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21480.1923051097831196No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21030.18827637144967435No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAAATGTGACAT20770.1859486559681282No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC19970.17878645448644775No Hit
GATTTCACTCTCACCATCACCAGTCTGCAGGTTGAAGATTTTGCAACGTACTACTGTCAACAGAATTACTACTAC19150.17144519796772534No Hit
CACCATCACCAGTCTGCAGGTTGAAGATTTTGCAACGTACTACTGTCAAC18930.1694755925602632No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAGGTTGAAGATT18500.16562590926385998No Hit
GTAGTAATTCTGTTGACAGTAGTACGTTGCAAAATCTTCAACCTGCAGACTGGTGATGGTGAGAGTGAAAT18410.16482016159717094No Hit
GTAGTACGTTGCAAAATCTTCAACCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA17800.1593589829673896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17790.1592694554488686No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17640.15792654267105352No Hit
ACGTACTACTGTCAACAGAATTACTACTACCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA17630.1578370151525325No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17340.15524071711542337No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17190.15389780433760827No Hit
GCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCATCCTCC16900.1513015063004991No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16790.15031670359676805No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16240.14539269007811276No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16170.14476599744846572No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA16060.14378119474473466No Hit
CCTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCATCCTCCCTGTCTGCATCTGTAGGAGA15950.1427963920410036No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15730.14082678663354148No Hit
GTTTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGTTC15580.1394838738557264No Hit
GAGTGAAATCTGTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA15430.13814096107791132No Hit
GCCCCTTACCTCCTCATCTATGCTGCATCCACTTTACATGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGT15420.1380514335593903No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACCATGTAAAGT15200.1360818281519282No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15130.13545513552228114No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14980.1341122227444661No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14980.1341122227444661No Hit
ATGTAAAGTGGATGCAGCATAGATGAGGAGGTAAGGGGCTTTCCCGGGTT14790.13241119989256697No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14740.13196356229996195No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14610.13079970455918888No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14550.13026253944806285No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14140.12659191118870164No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13740.12301081044786141No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13460.12050403992927326No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13340.11942970970702119No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13260.11871348955885316No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13080.11710199422547504No Hit
GCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCGAGG12950.115938136484702No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12700.11369994852167685No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12620.1129837283735088No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12540.11226750822534078No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAGGTTGAAGATTTTGCAACGTA12470.11164081559569372No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12400.1110141229660467No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12340.11047695785492066No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12310.11020837529935763No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12200.10922357259562658No Hit
GATGAGGAGGTAAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAAT12060.1079701873363325No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11950.10698538463260145No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11940.10689585711408044No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCACTGAACCTTG11930.10680632959555944No Hit
GTGCCAAATGTGACATCCAGATGACCCAGTTTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA11780.10546341681774436No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11580.10367286644732425No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11500.1029566462991562No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11480.10277759126211419No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCC11430.10232995366950917No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11430.10232995366950917No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11300.10116609592873609No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11270.10089751337317308No Hit
GGGTAGTAGTAATTCTGTTGACAGTAGTACGTTGCAAAATCTTCAACCTG11250.10071845833613106No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAAACTGGGTCATCTGGATGT11230.10053940329908906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2950.048.666451
ATGTCGT507.0635E-848.19637737
GACAGGT2700.040.5124551
GTATCAA33050.038.060871
CCGTCTT2150.037.1799647
CGTATGC2450.036.66641
CCCTAAG3450.035.71656428
TCGTATG2450.035.2254740
ACGAGAC3500.035.154322
CGGCTAA400.002474075434.8408549
ATGCCGT2500.034.66205644
GTCGTAT501.8661084E-434.4850939
TGTCGTA501.8760399E-434.45395738
TATCAAC36850.034.134482
CTTATAC4100.032.5149541
ATCTCGT1700.032.4009337
TAAGACA3300.032.2059231
ATCAACG39350.032.0526963
TCAACGC39450.031.8819474
CGAGACC3800.031.48377823
Produced by FastQC (version 0.11.5)