FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116975
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42730.38255108664025606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42450.3800443161216679No Hit
GTACTACTGTCAACAGAATTACTACTACCCTCGCACTTTTGGCCAGGGGA32920.2947245909711498No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32770.29338167819333466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28950.25918216611831063No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28550.2556010653774704No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28450.25470579019226036No Hit
CATCTATGCTGCATCCACTTTACATGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA26880.2406499697844625No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26100.23366682333982408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24190.21656706730231204No Hit
GAATTACTACTACCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT23170.2074352604131695No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGTTCCTATTTAAATTGGTATCAGCAGAAA22820.20430179726493433No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22490.20134738915374112No Hit
GGTAAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATAGGAACTA22360.20018353141296805No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGG22290.199556838783321No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22170.19848250856106894No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21730.19454329774614473No Hit
GCATAGATGAGGAGGTAAGGGGCTTTCCCGGGTTTCTGCTGATACCAATT21030.18827637144967435No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20460.18317330289397704No Hit
CACCATCACCAGTCTGCAGGTTGAAGATTTTGCAACGTACTACTGTCAAC20400.182636137782851No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20250.18129322500503592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20210.1809351149309519No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20000.17905503704201078No Hit
GGCCAAAAGTGCGAGGGTAGTAGTAATTCTGTTGACAGTAGTACGTTGCA19770.17699590411602767No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA19540.17493677119004455No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19450.1741310235233555No Hit
CAGTAGTACGTTGCAAAATCTTCAACCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA18860.1688488999306162No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAAATGTGACAT18150.1624924461156248No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18100.16204480852301978No Hit
GTAGTAATTCTGTTGACAGTAGTACGTTGCAAAATCTTCAACCTGCAGACTGGTGATGGTGAGAGTGAAAT17980.1609704783007677No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAGGTTGAAGATT17860.15989614807851563No Hit
GATTTCACTCTCACCATCACCAGTCTGCAGGTTGAAGATTTTGCAACGTACTACTGTCAACAGAATTACTACTAC17820.15953803800443161No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC17500.15667315741175944No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17030.1524653640412722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17010.15228630900423018No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16860.1509433962264151No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16810.15049575863381007No Hit
ACGTACTACTGTCAACAGAATTACTACTACCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA16230.14530316255959175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15800.14145347926318852No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15720.14073725911502047No Hit
GCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCATCCTCC15460.13840954363347435No Hit
GTAGTACGTTGCAAAATCTTCAACCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA15280.13679804830009623No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14870.13312742004073502No Hit
GAGTGAAATCTGTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA14670.1313368696703149No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14650.1311578146332729No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14650.1311578146332729No Hit
CCTCTGGCTCCGAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCATCCTCCCTGTCTGCATCTGTAGGAGA14580.13053112200362588No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14280.1278452964479957No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACCATGTAAAGT14240.12748718637391168No Hit
GCCCCTTACCTCCTCATCTATGCTGCATCCACTTTACATGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGT13970.12506994337384453No Hit
GTTTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGTTC13840.12390608563307146No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13570.12148884263300433No Hit
ATGTAAAGTGGATGCAGCATAGATGAGGAGGTAAGGGGCTTTCCCGGGTT13560.12139931511448332No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13540.1212202600774413No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13510.1209516775218783No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13150.1177286868551221No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13100.11728104926251708No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12850.11504286129949194No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12530.11217798070681977No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12220.10940262763266859No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12200.10922357259562658No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12140.10868640748450055No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12140.10868640748450055No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACT12020.1076120772622485No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCACTGAACCTTG11890.10644821952147542No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11720.10492625170661833No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11640.10421003155845028No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11580.10367286644732425No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAAACTGGGTCATCTGGATGT11510.10304617381767722No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAGGTTGAAGATTTTGCAACGTA11220.10044987578056805No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11180.10009176570648402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)