FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088862
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG40710.37387657940124647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37540.3447636155913238No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC25590.23501600753814533No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22980.2110460278713005No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG21810.20030086457237004No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21450.19699466048039146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20280.186249497181461No Hit
GTATAATAACTGGCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20250.18597398017379613No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC20190.18542294615846638No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18690.1716470957752222No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18130.1665041116321444No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17430.1600753814532971No Hit
CGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGG16220.1489628621441468No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15890.1459321750598331No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA15890.1459321750598331No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA15870.1457484970547232No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15810.14519746303939343No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15710.14427907301384382No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15640.14363619999595909No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA15280.1403299959039805No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15120.1388605718631011No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG15060.13830953784777134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14910.13693195280944692No Hit
GGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGACACGTTGTA14680.13481965575068283No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGG14150.1299521886152699No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14010.12866644257950044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13940.1280235695616157No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG13790.1266459845232913No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13630.1251765604824119No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC13620.12508472147985697No Hit
GTGTCGGACATGACCAGGGACACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACC13400.12306426342364782No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12450.1143395581809265No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC12330.11323749015026699No Hit
GACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCG12290.11287013414004712No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA11870.10901289603273875No Hit
GTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTG11730.1077271499969693No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG11440.10506381892287545No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT11440.10506381892287545No Hit
GGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCGCC10960.1006555468002373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)