FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730743
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23050.3154323749936708No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21180.289841982749065No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20820.284915490124435No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16400.22442910845536665No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16130.22073423898689415No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15980.21868153372663168No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15650.21416558215405415No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15030.2056810670783025No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14980.2049968319915483No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14650.20048088041897083No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13350.1826907681633625No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG12710.17393255905290916No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGTTGAAGC12490.17092192467119083No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGTTGAAG12270.1679112902894725No Hit
GATTATTACTGCAGCTCATATGGAGGCAGCTTCAACCCTTATGTCTTCGG12050.16490065590775416No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12020.16449011485570167No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11960.16366903275159667No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11880.16257425661278999No Hit
GTGTTGGTACCAGGAGACAGAGTTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA11810.16161632749133414No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11730.1605215513525275No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC11490.15723722293610748No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGGTTGAAGCTGCCTCCATATGAG11320.15491082364114334No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCCCAGGGAGA11170.15285811838088081No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT11070.15148964820737248No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATTATTTATGACGTCA10850.14847901382565415No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT10820.14806847277360166No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC10660.14587892049598833No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10310.14108927488870915No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9750.1334258419170625No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9320.12754142017097667No Hit
GAGTTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAC8880.12152015140754001No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTCTGTCTCCTGGTACCAACACCACCCAGGCAAAGC8670.1186463640431725No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTCTGTCTCCTGGTACCAACACCA8390.11481464755734917No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8360.11440410650529667No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGGAGGCAGCTTCAACCCTTATGTCTTCGGAACTGGGACCAAGGT8280.11330933036649No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA8260.11303563633178833No Hit
CCCCTGAGGGCCGCTTACTGACGTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA8250.1128987893144375No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8180.11194086019298166No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8080.11057239001947333No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7910.10824599072450916No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7830.1071512145857025No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG7830.1071512145857025No Hit
GTTATAACTCTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA7600.10400373318663333No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7360.10071940477021334No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7340.10044571073551166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)