FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402064
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT42371.0538123283855307No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA17390.4325182060567472No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCAGTGATGGCCAGGGAGG15160.377054399299614No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC12380.30791117831986947No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT11670.2902522981415894No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11290.28080106649687614No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10690.26587806916311835No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG9380.23329619165108045No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC8400.20892196267260935No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT8310.20668351307254565No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC8310.20668351307254565No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC8120.20195789725018903No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7030.17484778542719567No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6860.17061960284929764No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6610.1644016872935652No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT6410.15942735484897927No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6410.15942735484897927No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC6060.15072227307095387No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6010.1494786899598074No Hit
CATCTATAGTGATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC5920.14724024035974373No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG5910.14699152373751442No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCTTACAGGATTACTTCCGATGTTGGAC5490.13654542560388394No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC5180.12883521031477574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.12659676071471207No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5070.12609932747025349No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC5040.1253531776035656No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5010.12460702773687772No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG5010.12460702773687772No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC4960.12336344462573123No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC4870.12112499502566756No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT4860.12087627840343824No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4800.11938397867006247No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4800.11938397867006247No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACATTCAGGC4640.11540451271439374No Hit
GGCCATCACTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG4620.11490707946993513No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG4600.11440964622547653No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGTTTATGTCTTCGGAACTGGGAC4590.11416092960324724No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC4570.11366349635878864No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA4570.11366349635878864No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC4560.11341477973655935No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA4450.11067889689203708No Hit
GCATGGGATGACAGCCTGAATGTTTATGTCTTCGGAACTGGGACCAAGGT4270.10620199769190974No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG4250.10570456444745115No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG4120.1024712483584703No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA4090.1017250984917824No Hit
CTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAATCCTGTAAGCTGGTACCAGCAGCTCCCAGGAACGGCCCC4070.10122766524732381No Hit
CCCCTGAGGGCCGCTCATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCTTA4030.10023279875840663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5550.077.9058270
CGTCTTA307.758661E-659.0836248
AATCGCG601.8189894E-1256.65532336
TCGCGTT252.6739674E-454.73735438
ATCGCGT501.2387318E-954.5348337
CCGTGTT607.6397555E-1152.97141647
CCGTATT200.006707104452.97141647
ACGATTA200.007390924751.68692840
CGTGTTC351.9329915E-550.64310548
TGACGTC352.0608222E-550.09798445
CTTCGCA200.00849966649.8915323
TAATCGC750.049.7364435
CGCGTAT352.34111E-549.02960639
CGTTTGG506.834489E-848.38466641
CCGTCGT451.5822789E-647.085747
CTAATCG1050.045.12901734
CTCTTAT3050.044.6892781
GCGTATG704.474714E-1044.3030840
TATACAC12500.044.1431543
CTTATAC12300.044.0556681
Produced by FastQC (version 0.11.5)