FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402064
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117702.9273946436388236No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA15920.39595686258904056No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCAGTGATGGCCAGGGAGG12180.3029368458752835No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC10860.2701062517410163No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT9650.24001154045127146No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8850.2201142106729277No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC8690.21613474471725894No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC8330.20718094631700426No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG7480.18604003342751402No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC7420.18454773369413824No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT7330.18230928409407457No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6300.156691472004457No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6240.1551991722710812No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6220.15470173902662263No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT6200.15420430578216404No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6200.15420430578216404No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC6110.15196585618210037No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC5640.1402761749373234No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG5450.13555055911496677No Hit
CATCTATAGTGATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC5340.1328146762704445No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC5180.12883521031477574No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5040.1253531776035656No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCTTACAGGATTACTTCCGATGTTGGAC4820.11988141191452108No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC4740.11789167893668669No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG4700.11689681244776952No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4630.11515579609216442No Hit
GGCCATCACTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG4540.11291734649210075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4510.11217119662541286No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGTTTATGTCTTCGGAACTGGGAC4400.1094353137808906No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC4390.1091865971586613No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT4250.10570456444745115No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACATTCAGGC4200.10446098133630467No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA4200.10446098133630467No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC4190.10421226471407537No Hit
CCCCTGAGGGCCGCTCATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCTTA4160.10346611484738748No Hit
CTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAATCCTGTAAGCTGGTACCAGCAGCTCCCAGGAACGGCCCC4100.1019738151140117No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC4080.1014763818695531No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC4070.10122766524732381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGC150.0027185566.54214523
CGGCCAA200.003732020761.42587768
ACGCTTG200.00855244749.8134426
AAACTAG450.00920511548.88412570
GACAGGT1000.043.1985321
GCTTGTA451.2476281E-436.921821
GGTATCA8150.036.287581
CCAGTAC400.003121938833.2213673
TAATAGC400.00312765833.208965
ATAGCGG400.00312765833.208967
GTATCAA17650.032.947231
GCCAGAG6400.032.08020870
GTGGTAT2900.030.9379391
CGGGAAT450.005542459429.54481317
ACAGGTT902.7640635E-729.5374571
TGGTATC3050.029.4200672
TATCAAC19950.028.4860952
TCGTAAA500.006676725628.4395449
GTCGTAA500.0067904528.3413748
CGGGACC606.7694735E-427.6741334
Produced by FastQC (version 0.11.5)