FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372749
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGACGCTCACAGAGCTCAGCTTCAGGGAGA54900.39992744485699866No Hit
GTCTATGGTGGGTCCTTCAGTGGTTCCTTCTGGAGCTGGATCCGCCAGTC51930.37829202570899706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45710.3329814845976941No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36310.2645057472269148No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32690.2381353036862529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32460.23645983351654234No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31380.22859240837181452No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30080.21912235958649398No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28250.20579144475792732No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA27490.20025510854497072No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGCCTATTTAAATTGGTATCAGCAAAAA26380.1921691438128893No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26040.1896923618228824No Hit
GTATATTACTGTGCGAGGAGGGCTAGTGGCTCCGTCCGCTTCCACTTCTA24830.1808779317996225No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24600.17920246162991196No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT23520.1713350364851841No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGC23450.17082511078135917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22130.1612093689378029No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21640.15763988901102824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21510.15669288413249619No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21380.15574587925396413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21350.1555273396666106No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21230.15465318131719635No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20840.1518121666816002No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC20770.15130224097777525No Hit
GGGTTCCAGTGCCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT20050.14605729088129002No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTT20010.14576590476481863No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19910.14503743947364012No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19760.14394474153687237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19440.14161365260510114No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT19400.14132226648862975No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19060.13884548449862286No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18930.1378984796200908No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18920.13782563309097293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGCA18600.13549454415920173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17870.13017674753359865No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17810.12973966835889153No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGGCACTTACTACTGTCAACAGGGTTCCAGTGCC17740.1292297426550666No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGGTGGGACCCCA17180.12515033702446698No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16940.12340202032563856No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16380.11932261469503892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15870.11560744171002857No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15860.1155345951809107No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15590.11356773889472874No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15180.11058103120089688No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15080.10985256590971838No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14790.10774001656530072No Hit
GTAGTAAGTGCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14710.10715724433235793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14700.10708439780324007No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14660.10679301168676866No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14170.10322353175999399No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAATAGTCCTGCGCCCCACTGCTGTAGCTGCAC14120.10285929911440475No Hit
CTGGAACCCTGTTGACAGTAGTAAGTGCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13880.10111098241557634No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13870.10103813588645848No Hit
GTCATGGACTTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13770.10030967059527998No Hit
GTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13770.10030967059527998No Hit
GTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGGCGCTA13740.10009113100792642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAG400.00707732252.26101770
TACTGAT17900.048.66016870
GTGGTAT2750.048.384751
GTACGTA305.793681E-446.8064854
TGGATTG18500.043.31543770
GTATCAA27400.040.218751
GACAGGT2450.038.991371
TACGTAT400.002368123735.15368755
TATCAAC32450.033.942412
ATCAACG32400.033.7670563
TCAACGC32800.033.1401984
CAACGCA33450.032.697575
AACGCAG34250.032.630216
TCTACTG15500.032.31430468
TGTCTAC15450.031.46872366
ATAAACG652.5232528E-531.4608973
CGTATTC450.00415406831.34433657
ACGCAGA35650.031.2532227
TCCGCGA15850.031.18879517
GGTACGT450.00427032731.1680553
Produced by FastQC (version 0.11.5)