FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674227
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22130.32822773338949646No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20500.3040518994344635No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGAACAGAATTCT19940.29574609144991226No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16910.2508057375335013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16380.24294488354812252No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15290.22677822157819252No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14820.21980727559115848No Hit
GCATAATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14160.21001828760936597No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13800.20467883961929736No Hit
GCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGC12920.19162685564357404No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12790.1896987216471604No Hit
ACATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGAGAATTCTGTT12710.18851217764936734No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12410.1840626376576435No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12240.1815412316623333No Hit
AGTCAGGACATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGTCCCTAAGCGCCTGATCTATG12140.180058051665092No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA12120.17976141566564377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12020.17827823566840248No Hit
ACTTATTACTGTCTACAGCATAATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA11910.17664673767143707No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11780.1747186036750234No Hit
ATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA10790.16003512170233467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10550.15647548970895558No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10410.1543990377128178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10200.1512843597186111No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9890.1466865017271631No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGAGAATTCTGTTCCAGATCCACTGCCG9860.14624154772799072No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9790.1452033217299218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9720.14416509573185293No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAAAGTC9590.14223696173543926No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9560.14179200773626685No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9430.1398638737398532No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9310.13808405774316365No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8980.1331895637522674No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8890.13185470175475025No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8660.12844338776109532No Hit
CAGTAATACATGGCCGTGTCGTCAGATTTCAGGCTGCTCAGCTCCATGTA8640.12814675176164705No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8540.12666357176440576No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.12607029976550924No Hit
GTCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT8360.12399384776937143No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT8320.12340057577047492No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8130.12058253377571648No Hit
CTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATTGCTAA8130.12058253377571648No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7900.11717121978206152No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7810.11583635778454437No Hit
GTCTACAGCATAATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC7780.115391403785372No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7670.11375990578840658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7660.11361158778868245No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7560.11212840779144116No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7500.11123849979309638No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGAACAGAATTCTCTCTCA7280.10797550379916555No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7240.10738223180026904No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7060.10471250780523475No Hit
GTCTCCATCTGCCATGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7030.10426755380606234No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6910.1024877378093728No Hit
GAATTCTCTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATGTTTAT6870.10189446581047629No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6850.10159782981102805No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTG6800.1008562398124074No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6800.1008562398124074No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6790.10070792181268327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002353016869.01549539
TCGTATG851.8189894E-1244.71399340
ATCGCGT405.0463263E-543.0573237
ACGAGAC1550.041.9169622
ATCTCCG1800.039.87985610
GTGGTAT1750.039.0775761
TCTCCGA1850.038.8020211
TATACAC2100.037.4465643
GTATCAA15300.036.6511881
CACGAGA1700.036.2069121
CCCACGA1700.036.20422419
GTATGCC1250.035.99855842
CGAGCCC1900.035.98716415
GACATCT2050.035.0476526
CTTATAC2050.035.026851
CGTATGC1201.8189894E-1234.58802441
TCAACGT450.002692612134.24209668
GCCCACG1900.034.1903418
TACACAT2200.034.187815
ACGGGTT400.002713883834.1878055
Produced by FastQC (version 0.11.5)