FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957866
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27520.28730532245637697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24840.25932646111251473No Hit
GTATTACTGTCATCAGAGTAGTAGTTTACCTCGAACTTTTGGCCAGGGGA24140.25201854956747605No Hit
GAGTAGTAGTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22270.23249598586858705No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT22150.2312432010322947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20490.2139130107969173No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19020.19856639655233613No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT18500.19313766226173598No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18050.1884397191256397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17610.18384617472590112No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17260.1801922189533818No Hit
CAGTAATACGTTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA16460.17184032004476618No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15770.1646368072360852No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14450.15085617403686946No Hit
CTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC14320.1494989904642194No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14110.14730661700070782No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13840.14448785111905008No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA13180.13759753451944218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12660.13216880022884203No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12520.1307072179198343No Hit
GATTTCACCCTCACCATCAATAGCCTGGAACCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTAGTAGTTTA12520.1307072179198343No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT12420.12966323055625736No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11570.12078933796585326No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11540.12047614175678016No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11480.119849749338634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11350.11849256576598396No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11090.11577819862068389No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11030.11515180620253773No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10820.11295943273902612No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10590.11055826180279914No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10530.10993186938465296No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAC10420.10878348328471832No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10370.10826148960292985No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9780.10210196415782584No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9750.10178876794875276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT200.006574123653.24635351
GGTATCA11700.043.040921
TCGCTAA4000.041.45240831
CGCTAAT3850.040.50953732
ATGCCGT4000.040.0332144
ATCTCGT3250.040.0116537
GTATCAA33250.039.882431
TGGTATC3500.038.365912
ACTCGCT4600.038.1300429
GTGGTAT3700.037.201321
GTATGCC4650.036.5248842
ACACTCG4800.036.4647727
CTTATAC5000.036.257671
ATCTCCG5050.035.89119310
CACTCGC4800.035.79913328
TATACAC5450.035.726053
CCCACGA4900.035.63106519
CCGTCTT4450.035.4579447
TCGTATG4600.035.23821640
CTCGCTA4900.035.16805330
Produced by FastQC (version 0.11.5)