FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957866
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46760.4881684912085824No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26280.2743598791480228No Hit
GTATTACTGTCATCAGAGTAGTAGTTTACCTCGAACTTTTGGCCAGGGGA22290.23270478334130243No Hit
GAGTAGTAGTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21780.22738044778705999No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19020.19856639655233613No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT18870.1970004155069707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18730.19553883319796297No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17880.1866649406075589No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15580.16265323124528902No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15300.15973006662727354No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.15691130074561577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14930.15586731338203882No Hit
CAGTAATACGTTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA14670.15315294623673875No Hit
CTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC13230.13811952820123066No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12720.13279519264698822No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12670.13227319896519973No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12180.12715766088367267No Hit
GATTTCACCCTCACCATCAATAGCCTGGAACCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTAGTAGTTTA12170.12705326214731497No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12110.1264268697291688No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11910.1243388950020149No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT11910.1243388950020149No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA11730.12245971774757639No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11690.1220421228021456No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11550.12058054049313786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11470.11974535060227633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10930.11410781883896078No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10900.1137946226298877No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10670.1113934516936607No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10460.1092010782301491No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10150.10596471740306056No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10060.10502512877584129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCA400.006943260352.51170370
GTGGTAT4250.042.6225931
GGTATCA15100.039.9880831
TGGTATC4700.039.9587332
GTATCAA38250.037.6236461
TATCAAC41700.034.3410342
CCGCCTA551.9140719E-434.3353366
ATCAACG43750.032.474793
TCAACGC44100.032.2069634
AACGCAG44650.032.1103326
CAACGCA44650.031.9602835
CGCAGAG46550.030.5838188
ACGCAGA47250.030.3434127
AGAGTAC47350.030.06709111
CTAGTTG450.005330529529.78557416
GCAGAGT48500.029.2850919
ACGGGTA3650.028.45950916
TACGTAT1000.00719807928.0062470
ACGGGCT1104.859612E-827.41626716
CGATTAG808.671732E-526.24869246
Produced by FastQC (version 0.11.5)