FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167660
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC52920.4532141205487899No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGG40140.34376445198088484No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35880.3072812291249165No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31030.26574516554476474No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30420.2605210420841683No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28870.24724663001216107No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC27160.23260195604884984No Hit
GCAGTAATACTTATCCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA26970.23097477005292635No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG26720.22883373584776392No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGGATAAGTATTA26680.22849117037493794No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25510.2184711302947776No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG25270.21641573745782164No Hit
GACTATTACTGTCAATCAACAGACAGCAGTAATACTTATCCCGTATTCGG23610.20219927033554289No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA22560.19320692667386055No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG22410.19192230615076306No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21970.18815408594967714No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21250.18198790743880924No Hit
GTCTCTGGTGTCTCCATCACCAATAGTAGTTACTACTGGGGCTGGATCCG21240.18190226607060275No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20610.17650685987359335No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20570.17616429440076734No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19980.17111145367658392No Hit
CTATTACTGTCAATCAACAGACAGCAGTAATACTTATCCCGTATTCGGCG19890.17034068136272545No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19850.16999811588989946No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19230.16468835106109656No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC19210.16451706832468355No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19060.16323244780158608No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19040.16306116506517307No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18850.1614339790692496No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18760.1606632067553911No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGCTGATATATAAAGACATTGAGAGGCC18610.15937858623229365No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18550.15886473802305465No Hit
GTACCAGCCACTGCTACAGGCTCACTCGCACAGTAAAACACAGCCGTGTC18320.1568949865543052No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18260.1563811383450662No Hit
GGATAAGTATTACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTC17950.15372625593066475No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17770.15218471130294778No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT17690.15149958035729577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17500.1498723943613723No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17270.14790264289262287No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16810.14396313995512391No Hit
TACTTATCCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT16280.1394241474401795No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15940.13651234092115855No Hit
GTAAAACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA15890.13608413408012607No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15670.1342000239795831No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT15540.1330866861928986No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15420.13205898977442063No Hit
CAGTAATACTTATCCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT15350.13145950019697514No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA15210.13026052104208416No Hit
GGCCAGGCCCCTGTGTTGCTGATATATAAAGACATTGAGAGGCCCTCAGG14500.12417998389942278No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAG14200.12161074285322782No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14140.12109689464398883No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGGATAAGTATTAC14060.12041176369833684No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13960.11955535001627185No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13840.11852765359779388No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13540.11595841255159893No Hit
GTGTTGCTGATATATAAAGACATTGAGAGGCCCTCAGGGATCCCTGAGCG13430.11501635750132745No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC13350.11433122655567546No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13290.11381737834643647No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT13150.1126183991915455No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCAATAGTAGTTACTACTGGG13080.11201890961409998No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12950.1109055718274155No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12890.11039172361817652No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12540.10739427573094908No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT12530.10730863436274257No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12330.10559580699861261No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT12100.10362605552986315No Hit
GCTTGGTCCCTCCGCCGAATACGGGATAAGTATTACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTCTT12090.10354041416165666No Hit
CCCCTGTGTTGCTGATATATAAAGACATTGAGAGGCCCTCAGGGATCCCT12080.10345477279345015No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12030.10302656595241766No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11950.10234143500676568No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTT11790.10097117311546168No Hit
GCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTG11690.1001147594333967No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG11680.1000291180651902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2550.066.5652870
GTGGTAT2450.051.615791
TCGTTAA554.4480228E-449.37932670
CGTATGC2400.041.7201541
TGGTATC3200.041.652682
GAGACTC4000.041.01011724
TCGTATG2450.040.8263240
TCTCGTA1950.040.5819538
CACGAGA4050.040.50208321
TCGACGT4000.040.20739729
AGACTCG4000.040.15745525
CGAGACT4100.040.00644323
GGTATCA8800.040.0038221
GTATCAA24550.039.9555741
GACGTCA4050.039.7433931
CGACGTC4050.039.7229330
CCCACGA4150.039.52612719
CTCGACG4000.039.33505228
TATACAC4200.039.0589263
GATAACG350.001412885539.05223510
Produced by FastQC (version 0.11.5)