FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167660
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41860.3584947673124026No Hit
GTATTACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC38920.33331620505969206No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34370.2943493825257352No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGG32880.281588818662967No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30900.26463182775808025No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28550.24450610622955313No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC27470.23525683846325132No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG25350.21710086840347362No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG24740.2118767449428772No Hit
GCAGTAATACTTATCCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA24030.20579620780021582No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG23550.2016854221263039No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23500.20125721528527138No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21610.18507099669424318No Hit
GACTATTACTGTCAATCAACAGACAGCAGTAATACTTATCCCGTATTCGG21090.18061764554750526No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20350.17428018430022438No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20260.17350941198636588No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20150.1725673569360944No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGGATAAGTATTA20040.17162530188582292No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19910.17051196409913844No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC19760.16922734357604097No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19420.16631553705702004No Hit
CTATTACTGTCAATCAACAGACAGCAGTAATACTTATCCCGTATTCGGCG19160.16408886148365107No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18890.1617765445420756No Hit
GTCTCTGGTGTCTCCATCACCAATAGTAGTTACTACTGGGGCTGGATCCG18470.15817960707740267No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18100.15501087645376224No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18010.15424010413990372No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16990.1455046845828409No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGCTGATATATAAAGACATTGAGAGGCC16900.1447339122689824No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16860.14439134679615642No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16770.14362057448229792No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16750.14344929174588492No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT16550.14173646438175497No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16190.13865337512632103No Hit
GTACCAGCCACTGCTACAGGCTCACTCGCACAGTAAAACACAGCCGTGTC16050.13745439597143005No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15830.13557028587088707No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15690.1343713067159961No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA15580.1334292516657246No Hit
TACTTATCCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT15570.1333436102975181No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA15400.13188770703800762No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15040.12880461778257368No Hit
CAGTAATACTTATCCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT14840.12709179041844373No Hit
GGATAAGTATTACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTC14480.12400870116300979No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAG14430.12358049432197729No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14370.1230666461127383No Hit
GTAAAACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA14340.1228097220081188No Hit
GTGTTGCTGATATATAAAGACATTGAGAGGCCCTCAGGGATCCCTGAGCG14090.12066868780295634No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14060.12041176369833684No Hit
GGCCAGGCCCCTGTGTTGCTGATATATAAAGACATTGAGAGGCCCTCAGG13960.11955535001627185No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13870.11878457770241337No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT13750.1177568812839354No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC13580.11630097802442492No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13090.11210455098230648No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12700.10876453762225305No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12600.10790812394018806No Hit
CCCCTGTGTTGCTGATATATAAAGACATTGAGAGGCCCTCAGGGATCCCT12570.10765119983556858No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12370.1059383724714386No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12330.10559580699861261No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12200.10448246921192814No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12090.10354041416165666No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCAATAGTAGTTACTACTGGG12060.10328349005703716No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGGATAAGTATTAC11940.10225579363855918No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT11910.10199886953393969No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11900.10191322816573319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2550.048.1896632
GTGGTAT2600.047.2811741
GGTATCA9800.045.6461641
GGGCGAA306.648907E-445.51635424
GTATCAA24700.037.603741
TATCAAC28250.032.7448042
ATCAACG28750.032.1684383
TCAACGC29200.031.5463524
CAACGCA29450.031.3930595
AACGCAG29700.031.2436756
ACCGCTC450.004319731631.09448252
ACGTACC1101.1896191E-931.02324313
TACGTAC1101.193257E-931.0152712
AACCGTG553.4783647E-431.0139438
GACAGGT2500.030.049811
GTGCTAG1151.900844E-929.693491
AATACGT1151.9208528E-929.66550810
CGCAGAG32000.029.1046478
ACGCAGA31900.029.0889427
TACGGAT8200.029.06887670
Produced by FastQC (version 0.11.5)