FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786180
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24040.3057823908010888No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9700.12338141392556412No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8810.11206085120455875No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7890.10035869648172173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4400.053.985071
TCGCTAA400.00805264150.57805370
GGTATCA18500.042.1875571
TGGTATC5600.041.1839452
GTATCAA42700.037.1917231
TATCAAC52150.030.3717382
AACGCAG52550.030.1175446
AGGCTCG900.005166598629.97218370
ATCAACG53400.029.7764423
TCAACGC53800.029.3585284
ACGCAGA55150.028.7591327
CAACGCA55150.028.697685
CGCAGAG55950.028.3479188
TAACGGC1850.027.81122236
AGAGTAC58100.027.29716511
GCAGAGT58700.027.0775999
CAGAGTA59350.026.78104410
TTAACGG1950.026.3595535
GTATGGA659.5329515E-426.102671
GACAGGT2800.025.4501041
Produced by FastQC (version 0.11.5)