FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537602
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGACA55260.35939079163528664No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53480.34781432386274214No Hit
GTCTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAGGGGTCGAGT49890.3244662793102507No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46580.3029392521601819No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTACTGCCTGCATATGAGCTGCAGTAATAAT44150.2871354225605846No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC43280.28147726134591394No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG41420.2693805028869629No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40240.2617062152624671No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40100.2607957065612558No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38690.2516255832133413No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG36870.2397889700975935No Hit
GTGTATTACTGTGCGAGACGGCCCATTCACTCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGT36230.23562664460634156No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36080.2346510995693294No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT34650.2253509035498133No Hit
ATAATAGACAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGAAGTAATTATAA34240.22268441378197998No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33940.22073332370795562No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTACTGC32540.21162823669584196No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG30060.19549922541724063No Hit
GATTATTACTGCAGCTCATATGCAGGCAGTAACACCTTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT29820.19393835335802112No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT28870.187759901456944No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27400.17819956009422463No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGC27210.1769638697140092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25800.16779374636609473No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25410.16525732926986308No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25110.16330623919583873No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24920.1620705488156233No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24790.16122507645021272No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGA24740.16089989477120867No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC24690.1605747130922046No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTACTGCCTGCATATGAGCTG24630.16018449507739974No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24560.15972924072679406No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTA24550.15966420439099324No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC23710.15420115218372504No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTCTGTCTCCTGGTACCAACAACA23640.15374589783311937No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23550.15316057081091206No Hit
CAGTAACACCTTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA23160.1506241537146804No Hit
GTTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA22570.14678700990243249No Hit
CCCCTGAGGGCCGCTTACTGACCTCAGAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA21900.1424295754037781No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21720.1412589213593635No Hit
GAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAC21520.13995819464334724No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20790.13521054212988795No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG20610.13403988808547335No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20390.13260908869785548No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20300.13202376167564817No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20300.13202376167564817No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAG19580.12734114549798972No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19070.1240242923721483No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19010.12363407435734344No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18960.1233088926783394No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18880.12278860199193288No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18630.12116269359691259No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18410.11973189420929473No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG18300.11901649451548581No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18290.11895145817968499No Hit
GTATTACTGTGCGAGACGGCCCATTCACTCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTC18240.11862627650068094No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17940.11667518642665659No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17780.11563460505384358No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17770.11556956871804278No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17670.11491920536003464No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT17630.1146590600168314No Hit
GTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCA17500.11381358765142084No Hit
CTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAGGGGTCGAGTCACCATATCCGTAGACACGTCCAAGAA17190.11179746124159567No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGTAACACCTTAGTCTTCGGAACTGGGACCAAGGTCAC17160.11160235223419324No Hit
CTCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT17120.11134220689098999No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17050.11088695254038432No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16960.11030162551817702No Hit
GGTTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGAGGTCAGTAAG16790.1091960078095632No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16650.10828549910835182No Hit
CACCTGCAGTGTCTCTGGTGCCTCCGTCACCAGTTATAATTACTTCTGGG16450.1069847723923356No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16450.1069847723923356No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACCGTCTCTGGGCTCCAGG16300.10600922735532342No Hit
CTATAATAGACAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGAAGTAATTATAA16090.10464346430350638No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16070.10451339163190475No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGTAACAC16010.10412317361709988No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15940.1036679192664942No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15940.1036679192664942No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15770.10256230155788039No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGG15760.10249726522207957No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15560.10119653850606335No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15490.10074128415545765No Hit
GTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC15410.10022099346905117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATT8950.057.0041431
TCTATTA9150.055.7581522
TACGGAT16200.053.99044470
GACGAAT200.006801448852.79230555
TACGTAT450.00975195248.18982370
GGTATCA15100.043.923391
GTGGTAT4400.043.28861
TATTATA12050.042.9036984
CTATTAT11850.042.7667353
TGGTATC4600.042.145892
TTATAGT12450.040.977556
TATAGTG12500.040.8109867
ATTATAG12550.040.6510355
CGTATGC2100.039.50871341
ATCTCGT1800.038.2330537
GTATCAA35050.038.1366651
TCTCGTA1900.038.0706638
ATAGTGG13600.038.0089958
ATGCCGT2200.037.84798444
ACACCTA13850.037.08339717
Produced by FastQC (version 0.11.5)