FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537602
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGACA52550.3417659446332666No Hit
GTCTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAGGGGTCGAGT48930.3182227910733727No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46500.3024189614737754No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44970.29246840209625113No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40510.26346219632908907No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTACTGCCTGCATATGAGCTGCAGTAATAAT38280.248959093445508No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38100.24778843940109338No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37570.24434151360365036No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36930.2401791881123984No Hit
GTGTATTACTGTGCGAGACGGCCCATTCACTCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGT36310.23614693529274808No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG36300.23608189895694726No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT35100.22827753866084982No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34250.2227494501177808No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31660.2059050391453705No Hit
ATAATAGACAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGAAGTAATTATAA31580.20538474845896404No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30420.19784053350606984No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC27880.1813213042126636No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27240.17715897872141165No Hit
GATTATTACTGCAGCTCATATGCAGGCAGTAACACCTTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27150.17657365169920436No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGC26340.17130570849933857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26270.1708504541487329No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT25910.16850914605990366No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTACTGC25650.16681820132908254No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25040.16285098484523303No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24980.16246076683042815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24220.15751800530956647No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG24030.15628231492935105No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTA23540.15309553447511126No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23390.15211998943809907No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAGGTGTTACTGCCTGCATATGAGCTG22520.14646182822342843No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22500.14633175555182681No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22190.14431562914200163No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTCTGTCTCCTGGTACCAACAACA21880.14229950273217648No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21870.14223446639637566No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGA21410.1392427949495383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21100.13722666853971313No Hit
GTTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA21080.13709659586811151No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20990.1365112688459042No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20550.1336496700706685No Hit
GAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAC20290.13195872533984737No Hit
CAGTAACACCTTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA20280.13189368900404655No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20060.1304628896164287No Hit
CCCCTGAGGGCCGCTTACTGACCTCAGAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA19980.12994259893002222No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC19000.12356903802154265No Hit
GTATTACTGTGCGAGACGGCCCATTCACTCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTC18270.11882138550808337No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18220.1184962038290793No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18090.11765073146366876No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17930.11661015009085576No Hit
GGTTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTCTGAGGTCAGTAAG17760.11550453238224195No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17710.11517935070323788No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGTAACACCTTAGTCTTCGGAACTGGGACCAAGGTCAC17540.11407373299462409No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAG17520.11394366032302246No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT17400.11316322429341273No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17210.1119275339131973No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16950.1102365891823762No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16760.10900089880216077No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16750.10893586246635996No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16620.10809039010094941No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16590.10789528109354696No Hit
CTCCTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT16440.10691973605653478No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16400.10665959071333153No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16160.10509871865411206No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15900.10340777392329095No Hit
GTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCA15900.10340777392329095No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15770.10256230155788039No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15690.1020420108714739No Hit
CTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAGGGGTCGAGTCACCATATCCGTAGACACGTCCAAGAA15660.10184690186407146No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15560.10119653850606335No Hit
GTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC15430.10035106614065278No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGTAACAC15400.10015595713325035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)