FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238874
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36090.2913129180207188No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31090.2509536885914145No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27880.22504306329780105No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25700.20744643926662434No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24250.19574226273212614No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23930.19315927204865063No Hit
GAGTTACATTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23530.1899305336943063No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23080.18629820304566888No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22670.18298874623246594No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGAGGGTCTGAGACAGATTTCA22350.18040575554899044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22110.17846851253638385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22100.17838779407752522No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20850.16829798672019913No Hit
GTCTGTATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA20790.1678136759670475No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20260.16353559764754122No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20150.16264769460009654No Hit
GTCTACAACCTGAAGATGTTGCAACTTACTACTGTCAACAGAGTTACATTACCCCTCGGACGTTCGGCCAAGGGA18740.1512663919010327No Hit
CCATAGATCAGGACCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATT18310.14779549817011253No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAACATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAAT17890.14440532289805097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17450.14085371070827218No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17440.1407729922494136No Hit
ACATTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA17430.14069227379055496No Hit
CCTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTA17120.1381900015659381No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16730.13504198167045237No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16440.1327011463635527No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15940.12866522342062225No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15900.12834234958518784No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15840.1278580388320362No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15580.12575935890171236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14930.1205126590759028No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14810.1195440375695995No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAACATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAAT14640.11817182376900315No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14630.11809110531014454No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA14240.1149430854146588No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14240.1149430854146588No Hit
GTCCCCATCCTCCCTGTCTGTATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC14170.11437805620264853No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATG13510.10905063791798036No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13440.1084856087059701No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13430.1084048902471115No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13420.10832417178825288No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13280.10719411336423236No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12840.10364250117445357No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12780.10315819042130193No Hit
GTGGATGCACCATAGATCAGGACCTTAGGGGCTTGCCCTGGTTTCTGCTG12740.1028353165858675No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12710.10259316120929166No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12520.1010595104909781No Hit
CCCCTAAGGTCCTGATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCA12490.10081735511440228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT353.3971992E-758.8621339
CTCTTAT2350.041.1970831
GACAGGT3750.039.1703761
ATGCCGT5950.038.1164144
CGTATGC6100.037.96741541
TCGTATG5900.037.36868340
GTATCAA48800.036.2570421
GGCAATC6100.036.05330733
ATCTCGT5400.036.02836637
CGAGACA8100.035.88859623
CTTATAC8350.035.1829721
TTATACA8350.035.178712
TATACAC8500.034.9506153
CCGTCTT6450.034.33474747
GCAATCT6050.034.17860434
AATCGCG502.0120041E-434.04640636
ACGAGAC8350.034.0096422
CCCACGA8350.033.5878119
GTATGCC7150.033.4580142
CACGAGA8500.033.01108621
Produced by FastQC (version 0.11.5)