FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666931
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62730.9405770611952361No Hit
GTATTACTGTCAGCAGTATGGTAGCACAACGGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10690.1602864464239929No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8520.12774934738376234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.004987451257.0885770
CTTACGC200.005926963454.65763560
GGTACCG508.5665306E-847.0243386
GCCGTTA504.5968773E-640.3307715
GTATCAA31750.040.252091
TATCAAC34500.036.829272
ATCAACG35950.035.140983
TCAACGC36250.034.7496534
CAACGCA36450.034.5563935
GGTATCA11750.034.3472481
AACGCAG37000.034.2242776
TACCGCG601.6115384E-533.5888148
CGCAGAG37850.033.2782178
AGAGTAC38100.032.96922711
TGCGGTA450.00369537132.09988857
GCAGAGT39600.031.6379479
CAGAGTA40300.031.08840410
ACGCAGA41100.030.8101777
TTAACGG450.004892397730.31126635
GAGTACG31200.029.7131812
Produced by FastQC (version 0.11.5)