FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301394
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45210.3473967145998829No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44850.34463045011733573No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG38680.29721975051367994No Hit
ATATTACTGTCAGCAGTATGATAATCTACCCCTCAGTTTCGGCGGAGGGA38240.29383876059056674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33430.25687839347653363No Hit
CAGTAATATGTTGCAATATCCTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATGCACTTCCA32770.25180690859186383No Hit
GTATGATAATCTACCCCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32170.2471964677876185No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31650.24320075242393926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30210.23213569449375054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30110.231367287693043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29610.22752525368950524No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29340.2254505553275949No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27500.21131187019457595No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA26750.20554881918926934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25800.19824895458254765No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25350.19479112397936366No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24630.1892585950142693No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24180.18580076441108534No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA22920.17611883872217024No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22720.17458202512075513No Hit
TTATCATACTGCTGACAGTAATATGTTGCAATATCCTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC21460.16490009943184No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20840.1601359772674532No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20100.15444976694221735No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19220.14768778709599092No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA19110.1468425396152126No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT18980.14584361077429278No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT18540.14246262085117958No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18410.14146369201025977No Hit
GTAATATGTTGCAATATCCTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATGCACTTCCA18400.14138685133018902No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18150.13946583432842014No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17980.1381595427672173No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG17980.1381595427672173No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17740.13631536644551917No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA17560.1349322342042456No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17200.13216596972169842No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16820.12924602387900974No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16770.12886182047865596No Hit
ACATATTACTGTCAGCAGTATGATAATCTACCCCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16590.12747868823738237No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16530.12701764415695785No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16500.12678712211674556No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAGGATATTGCAACATATTACTGTCAGCAGTATGATAATCTA16390.1259418746359673No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15950.12256088471285408No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15850.12179247791214651No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15570.11964093887016539No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG15400.11833464730896255No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14980.11510733874599084No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14910.11456945398549555No Hit
GCCCCTAACCTCCTGATCTACGATGCATCCGATTTGAAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGCAT14810.113801047184788No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14710.11303264038408047No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14120.10849904025990592No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14070.10811483685955214No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA13940.10711590801863233No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13940.10711590801863233No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13820.10619381985778327No Hit
ACCTTGATGGGACCCCTGTTTTCAAATCGGATGCATCGTAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG13750.10565593509728798No Hit
GATCTACGATGCATCCGATTTGAAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGCATCTGGGACAGATTTTA13650.10488752829658043No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13610.10458016557629742No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13480.1035812367353776No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13430.10319703333502384No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13180.10127601633325496No Hit
GGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTACGATGCATC13180.10127601633325496No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13170.1011991756531842No Hit
GTTCAGTGGAAGTGCATCTGGGACAGATTTTACTTTCACCATCAGCAGCC13110.10073813157275968No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13100.10066129089268891No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13070.10043076885247666No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13030.10012340613219364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT300.002544267367.6684770
CGACACT200.006732180752.92865858
GTACGGT200.00768034551.19399313
CGTATGC1300.047.81818841
TCGTATG1200.046.01136840
CGAGACG2400.042.6813423
GTATCAA30800.042.3389931
GCCGTCT1450.040.7033746
AATCGAG700.0014828238.66769870
CACGAGA2600.038.08195521
ACGAGAC2650.037.3648722
CCCACGA2650.037.3562519
TATACAC2950.037.0273323
ATGCCGT1700.036.6852344
ACGCTCA2800.036.61215227
GTGGTAT3550.036.541221
ATCTCGT1600.036.53614837
TATCAAC36000.036.3153952
GAGACGC2900.035.32384524
ATCTCCG2950.034.70778710
Produced by FastQC (version 0.11.5)