FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848434
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30430.35866077974244315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28970.3414526056240085No Hit
GAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT26490.31222228246392764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23110.2723841807376885No Hit
GTAGGAGACAGTGTCGCCATCACTTGCCGGGCAAGTCAGTCCATTAGCATCTATTTAAATTGGTATCGACAGACG22800.26873039034267837No Hit
ACCTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA21890.2580047475702294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21630.25494027820667253No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20370.24008938821405082No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17760.20932683037219157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17510.20638022521492538No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17050.20095847172555553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16750.1974225455368361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16320.19235438466633822No Hit
GTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15550.1832788407819583No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14570.17172814856547475No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14200.16736717293272077No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.16571707404465166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14050.16559920983836102No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14020.16524561721948908No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTA13980.1647741603943265No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13880.16359551833142No Hit
GTCCATTAGCATCTATTTAAATTGGTATCGACAGACGCCAGGGAAAGCCCCTAAACTCCTGATCTATGGTGCAT13620.16053104896786313No Hit
CTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGA13530.1594702711112473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12660.1492160851639609No Hit
GTCTCAGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12560.1480374431010544No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12260.14450151691233495No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12190.14367646746830043No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11990.14131918334248744No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11990.14131918334248744No Hit
GATCTATGGTGCATCTTTTTTACAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGCGGATCTGGGACAGATTTCA11880.1400226770732903No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11650.1373118003286054No Hit
CAGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11500.13554383723424568No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11120.13106499739520105No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10960.12917917009455068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCTTAAATGCA10890.12835412065051613No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10720.1263504291435751No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10680.12587897231841252No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC10540.12422887343034343No Hit
CTGTAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC10450.1231680955737276No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10380.12234304612969304No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGAGACAGTGTCGCCATCACTTGCCG10150.11963216938500815No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTT10030.11821779890952037No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9920.11692129264032323No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA9870.11633197160886999No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT9610.11326750224531312No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9560.11267818121385989No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9480.1117352675635347No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9440.1112638107383721No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9420.11102808232579083No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9400.11079235391320952No Hit
GTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGA9260.10914225502514044No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9240.10890652661255913No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9140.10772788454965267No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9100.10725642772449007No Hit
GATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGAT9050.1066671066930368No Hit
GTCTGCATCTGTAGGAGACAGTGTCGCCATCACTTGCCGGGCAAGTCAGT8940.1053706004238397No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTAGGAGACAGTGTCGCCATCACTTGCCGGGCAAGTCAGTCCATTAGCAT8930.10525273621754902No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8920.10513487201125839No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8910.10501700780496774No Hit
CCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCGTCTGTCGATACCAATT8810.10383836574206126No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCCTCC8740.10301331629802672No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8720.10277758788544542No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8710.10265972367915477No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8690.10242399526657349No Hit
CTCCTGATCTATGGTGCATCTTTTTTACAAAGTGGGGTCCCATCGAGGTT8590.10124535320366698No Hit
GTTTAGGGGCTTTCCCTGGCGTCTGTCGATACCAATTTAAATAGATGCTA8580.10112748899737635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)