FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848434
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33110.3902483870283369No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30690.361725249106No Hit
GAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT23500.2769808847830238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22730.2679053408986439No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22620.26660883462944673No Hit
ACCTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA20230.23843928932598177No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19500.22983520226676443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18480.21781305322511826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17290.2037872126765311No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16540.19494739720473248No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA15830.18657903855809646No Hit
GTAGGAGACAGTGTCGCCATCACTTGCCGGGCAAGTCAGTCCATTAGCATCTATTTAAATTGGTATCGACAGACG15820.18646117435180581No Hit
CTGTAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC14760.17396756848499706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14100.16618853086981428No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13990.16489202460061714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13790.16253474047480415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13780.1624168762685135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13350.15734871539801565No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13330.15711298698543433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13310.15687725857285306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13240.1560522091288185No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13050.15381278920929617No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTA12850.1514555050834832No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12190.14367646746830043No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC12150.14320501064313784No Hit
CTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGA12100.14261568961168458No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11910.14037626969216227No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11850.1396690844544184No Hit
GTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11590.13660461509086153No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCTTAAATGCA11260.1327150962832701No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10600.12493605866808732No Hit
GTCTCAGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10510.12387528081147148No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10150.11963216938500815No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10090.11892498414726425No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9990.11774634208435777No Hit
GAGTGAAATCTGTCCCAGATCCGCTGCCACTGAACCTCGATGGGACCCCA9920.11692129264032323No Hit
GTCCATTAGCATCTATTTAAATTGGTATCGACAGACGCCAGGGAAAGCCCCTAAACTCCTGATCTATGGTGCAT9560.11267818121385989No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9530.11232458859498794No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9500.11197099597611601No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9190.1083172055811059No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9170.10808147716852459No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8890.10478127939238643No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8870.10454555097980514No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8750.10313118050431737No Hit
GTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTTCGGCCAAGGGA8660.10207040264770154No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTACAGTGCATTTAAGACGTT8650.10195253844141089No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8570.1010096247910857No Hit
GATCTATGGTGCATCTTTTTTACAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGCGGATCTGGGACAGATTTCA8570.1010096247910857No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8540.10065603217221375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2950.046.030651
GGTATCA9500.043.9532171
GTATCAA26500.039.5841871
CCCGAAA400.001769528337.30186567
TATCAAC30900.033.6041372
ATCAACG32550.032.094063
TCAACGC32900.031.7470154
AACGCAG33300.031.6693176
CAACGCA33300.031.3656755
ACGCAGA34500.030.5677747
CGCAGAG35000.030.3248628
AGAGTAC35400.029.88465511
GCAGAGT35950.029.6178349
CAGAGTA39150.027.37019310
GTGCTAG1005.774709E-727.1580851
ACGCACA500.00841328727.1276728
TGTTAGA9700.026.51225970
AATGGTA2706.488335E-926.45770870
ACTGTTA1358.802541E-426.45770870
AAGTTAC1251.127808E-625.1516568
Produced by FastQC (version 0.11.5)