FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061217
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC128410.4194736929789688No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC122490.4001349789969153No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG112990.36910156973517394No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT105460.34450350955192005No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTGAGGC100520.32836613673581455No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG93660.3059567485741782No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTGAGG93640.30589141508099554No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA90600.29596072411723834No Hit
GTATATTACTGTGCGAGAGATTTTAAGTTGGACTTTGACTACTGGGGCCA86310.2819466898295678No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA85380.27890868239657624No Hit
GACTATTACTGCTCAGCATGGGACATCAGCCTCAGTGCTTATGTCTTCGG85120.2780593469852023No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG84100.274727338832889No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC83780.2736820029419672No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG81940.2676713215691668No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC77710.25385328776104404No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC72340.23631124484151236No Hit
GTATATGGTAGTACTAGTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT70660.23082323141417285No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG68640.22422454860272892No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG64900.2120071853775802No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC64410.2104065147946062No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC60880.19887515324787494No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC60110.19635981376034434No Hit
GCATGGGACATCAGCCTCAGTGCTTATGTCTTCGGAACTGGGACCAAGGT59230.19348514006030934No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAGAAT58770.19198246971710925No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTGAGGCTGATGTCCCATG57370.18740912519432631No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG49470.16160239538719404No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG49400.16137372816105489No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG48810.15944639011216782No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG45960.1501363673336454No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT45590.14892769770976708No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT45530.1487316972302192No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC45350.14814369579157569No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT45110.14735969387338435No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC44810.14637969147564514No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG44190.14435435318698414No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAA43970.14363568476197539No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT43480.14203501417900136No Hit
CACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAACTTGGCTGCAGCAGCACCAGGGCCACCCTCC43390.1417410134596796No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT41250.13475032968913997No Hit
CCGCCACACTCACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGA40390.1319409894822876No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAACTTGGCTG39070.12762897893223513No Hit
CTCCAGAGACAATGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGACGAGGACACGGCTGTATATTA38060.12432963752651316No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC37000.12086696238783465No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT35900.11727362026279092No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA35700.11662028533096479No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG35570.11619561762527778No Hit
GTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC34880.11394161211047762No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG34770.11358227789797326No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC34520.1127656092331906No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA33300.10878026614905117No Hit
CATAAGCACTGAGGCTGATGTCCCATGCTGAGCAGTAATAGTCAGCCTCG33140.10825759820359028No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG32940.10760426327176414No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC32800.10714692881948584No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG32440.10597092594219881No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG32390.10580759220924227No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA32230.10528492426378137No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGTATCTCAGAGAGATT31080.10152824840578109No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT31030.10136491467282456No Hit
TCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGGCAGGGCTG30920.1010055804603202No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG30860.10080957998077236No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC30810.10064624624781583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1900.046.30129670
GGTATCA24550.041.6853451
TCTGGAG35200.040.1797370
GTATCAA62250.035.774231
GTACGTA2150.034.94479469
AATGGTA18550.032.83235570
CAACGCT552.8587817E-432.07090854
TCGTCTC28000.031.9323320
TATCAAC69650.031.728792
CGTCTCT28250.031.65077621
GGTACGT2250.031.24494268
CGTCTCC25650.030.63100466
ATCAACG72900.030.3609073
ACGGTAG450.004969071630.21852517
TATACTA34800.030.186955
ATATACT34900.030.101934
TCAACGC74150.029.8949414
GCTATAC803.0976025E-629.748793
AACGCAG74650.029.693256
GTATCTG39750.029.68562169
Produced by FastQC (version 0.11.5)