FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061217
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC122620.4005596467026023No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC110230.36008554767597334No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG99530.3251321288232752No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT91230.29801872915249067No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTGAGGC85760.2801500187670459No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA83440.2725713335578628No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC79020.2581326315645052No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA78580.2566952947144877No Hit
GTATATTACTGTGCGAGAGATTTTAAGTTGGACTTTGACTACTGGGGCCA78180.25538862485083547No Hit
GACTATTACTGCTCAGCATGGGACATCAGCCTCAGTGCTTATGTCTTCGG76400.24957394395758287No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG75580.24689527073709575No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG75200.2456539343666261No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTGAGG73520.24016592093928654No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG73520.24016592093928654No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70380.22990856250961625No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC68230.22288521199248534No Hit
GTATATGGTAGTACTAGTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT67190.21948787034698947No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC66770.21811586699015456No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG63020.20586583701841457No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACTGAGGCTGATGTCCCATG57980.189401796736396No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG57890.18910779601707425No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC57520.18789912639319592No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC56590.18486111896020438No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAGAAT54180.17698843303169948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG52190.17048775046002945No Hit
GCATGGGACATCAGCCTCAGTGCTTATGTCTTCGGAACTGGGACCAAGGT46730.15265170682117601No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT44910.1467063589415582No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT44490.14533435558472332No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG42970.14036901010284472No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG42020.13726566917667057No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG41820.13661233424484445No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC40870.13350899331867033No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAA40600.13262699116070503No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT40350.13181032249592237No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT40120.1310589873243223No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG39590.12932764975498307No Hit
CACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAACTTGGCTGCAGCAGCACCAGGGCCACCCTCC39270.12828231386406125No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC38560.12596297485607846No Hit
GTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC37340.12197763177193906No Hit
CTCCAGAGACAATGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGACGAGGACACGGCTGTATATTA36840.12034429444237374No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36610.1195929592707737No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAACTTGGCTG36580.11949495903099976No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG36410.11893962433894756No Hit
CCGCCACACTCACCTGCGCTGGGAACAGCAACAATGTTGGCAACCAAGGA35990.11756762098211267No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG35200.11498694800139944No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC34430.11247160851386884No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT34050.11123027214339919No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA33600.10976026854679038No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT33390.10907426686837293No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC32680.10675492786039016No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG32570.10639559364788578No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC32380.10577492546265098No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA31330.10234491707056376No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC31290.10221425008419854No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGACAGACCGCCAC30760.10048291251485929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA16250.045.27711570
GGTATCA26550.041.4673961
TCTGGAG34300.036.020770
GTATCAA64900.033.7184371
TACGTAT1052.3945507E-433.052770
AAAGTCA14250.032.63519370
TATCAAC69750.030.7304862
ATCAACG72000.029.763353
TCAACGC72900.029.392064
AACGCAG73600.029.157226
CAACGCA73650.029.0913245
CGTCTCT27250.028.05110521
CGTCTCC25350.028.01128466
TCGTCTC27450.027.72250720
AGAGTAC78200.027.52668611
CCGATTA756.710738E-527.25949730
CGCAGAG78600.027.2592378
CAGAGTA79200.027.22288510
GTGCTAG5250.027.1841811
GTCTCCT26700.027.02908167
Produced by FastQC (version 0.11.5)