FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321572
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC97280.4190264183062166No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC83610.3601439024936552No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG76360.32891506272473997No Hit
CCCATATACAACACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC71640.3085840111786324No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCCACTACCCATATACAACACA71260.3069471892321238No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG70910.3054395900708658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70180.30229516896309916No Hit
GATTATTACTGTGTGTTGTATATGGGTAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT66480.2863576921155148No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT64260.2767952060069643No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCCACTACCCATATACAACACACAGTAATAAT63240.27240163130844103No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG62730.2702048439591794No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT56990.24548021771454862No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG55500.23906215271376463No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT55220.23785607338475825No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCTTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG50320.21674968512714662No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCTTTGGGAACAAAG49670.21394985811338177No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48200.2076179416360983No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG47950.20654108509234262No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC46560.20055376270906095No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT46410.1999076487828075No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA44860.19323113821152219No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGCGCGTGGAGCCTGG44800.19297269264102082No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC44620.19219735592951673No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG44250.19060360824475828No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC44200.19038823693600715No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCTTTGGGAACAAAGCTGC43860.1889237120364994No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG43470.1872438158282405No Hit
GTGTTGTATATGGGTAGTGGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG43430.18707151878123962No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT43270.18638233059323597No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA42920.18487473143197797No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42130.18147186475370997No Hit
CTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCGCGCTCATCTACAGCACAAACA42010.18095497361270724No Hit
CCCCAGGCCAGGCTCCACGCGCGCTCATCTACAGCACAAACACTCGCTCT41640.17936122592794881No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC41220.17755210693443926No Hit
GTAGATGAGCGCGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG40950.1763891018671831No Hit
CCCAAAGGCCACTACCCATATACAACACACAGTAATAATCAGATTCATCA40080.17264164109491328No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC39280.16919570015489505No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA38000.1636821946508659No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG37890.16320837777161337No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG37670.16226074401310836No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT36520.15730720391183217No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA36390.1567472385090792No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35990.1550242680390701No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG35520.15299977773680937No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA33610.14477259374251586No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA33350.14365266293700993No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33150.14279117770200536No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32920.14180046968175014No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC32690.1408097616614949No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC32550.1402067219969917No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC32090.1382253059564812No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG31620.13620081565422051No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC31370.1351239591104648No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31170.13426247387546025No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT30470.1312472755529443No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA30360.1307734586736918No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG30160.12991197343868724No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC29700.12793055739817677No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC29380.12655218102216945No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG28850.12426924514940738No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC28730.12375235400840465No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28590.12314931434390146No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC28250.12168478944439372No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC27360.11785118014862343No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG26990.116257432463865No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAAGATGGAGCCAGAGAAGCGATC26880.1157836155846125No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT26850.11565439279936181No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26650.11479290756435725No Hit
ACCCATATACAACACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT26560.1144052392086052No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAG26530.11427601642335453No Hit
ATACAACACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA26400.11371605102060156No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGT26260.11311301135609837No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT25660.11052855565108469No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA25590.1102270358188331No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25010.10772872863731989No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC24910.1072979860198176No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCCACTAC24830.10695339192581578No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC24790.10678109487881488No Hit
CTGTAGATGAGCGCGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG24710.10643650078481304No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA24420.10518734719405644No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCCA24020.10346437672404732No Hit
GGGCAGCTTTGTTCCCAAAGATGGAGCCAGAGAAGCGATCAGGGACCCCA23820.10260289148904278No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAAGATGGAGCC23550.10143988642178663No Hit
GCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC23440.1009660695425341No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGTTGTATATGGGTA23430.10092299528078388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)