FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784789
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA78940.4422931786334407No Hit
GTATATCACTGTGCGAGCGCGGACTTGCCTAAATACGATTTTTGGAGTGGTCCCACGGCATTTGACGACTGGGGC53150.2977943050971291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51700.28967009545666184No Hit
GTATTACTGTCAGCAGTATGGTAGCTCAGTGGGGACGTTCGGCCAAGGGA46000.25773354721482483No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44650.25016962789438973No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG39410.22081041512470098No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA35210.19727822168334747No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA34650.19414059589116697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33310.1866327056027351No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCAGTGGGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA32650.18293478949052241No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31260.17514675404207444No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30820.17268147663393263No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA30010.1681431250416716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27450.15379969284884656No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26680.1494854573845984No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA26310.14741238320047917No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25680.14388255418427615No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25150.14091301548810534No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25060.1404087542000763No Hit
CCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA25030.14024066710406666No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24020.13458173487174116No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23500.13166822520757354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23380.1309958768235349No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23290.1304916155355059No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22460.1258412058792384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22310.12500077039919005No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGG22140.12404827685513525No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21960.12303975427907725No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21940.1229276962150708No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21890.12264755105505468No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21590.12096668009495799No Hit
GGGTTCCCTGGCCCCAGTCGTCAAATGCCGTGGGACCACTCCAAAAATCG20390.11424319625457126No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20360.1140751091585616No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20270.11357084787053258No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20210.11323467367851327No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20070.11245026723046814No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19950.11177791884642947No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19930.11166586078242302No Hit
GTATGGTAGCTCAGTGGGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19550.10953675756630056No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18610.10427002855799761No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18590.10415797049399117No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18520.10376576726996861No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCC18440.10331753501394282No Hit
CTCCAGGCACCTTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCGTGC18220.10208489630987193No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18010.10090828663780424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17910.10034799631777201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG150.002521079567.8297416
GTGGTAT4800.048.075721
TACGGAT24700.048.03457370
GTATCAA36500.035.981251
CGGTACT400.002192435835.71093459
GGTATCA18350.035.69271
CGATCAA501.6100639E-435.36330855
GTAAACG400.002796438533.98251327
CCGATAG400.002825828333.9101215
CTACGGA25800.031.5507669
TATCAAC41800.031.4075782
ATCAACG42950.030.3194773
AACGCAG42850.030.300056
TCAACGC42900.030.111764
GATATAC23500.029.7271333
CAACGCA43400.029.6825665
TGATATA23550.029.5299512
GCTTGTA1401.8189894E-1129.0878331
AGGTATA704.337249E-529.0617454
ACGCAGA45000.028.8523837
Produced by FastQC (version 0.11.5)