FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990781
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG69570.3494608397407852No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTC69490.3490589874024315No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC63620.3195730720757331No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCA60410.30344874699929325No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAG58470.29370382779421744No Hit
CACCTGCACCTTGCGCAGTGGCATAACTATTACTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC54790.2752186202299499No Hit
GACTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAG49220.2472396511720777No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49040.246335483410782No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG48380.24302020161936447No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG43970.22086809146762No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG43940.22071739684073735No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA43150.21674910499949518No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC42850.21524215873066901No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42290.21242919236219354No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT42000.2109724776356616No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG41860.2102692360435427No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG41230.20710464887900779No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA41020.20604978649082947No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACAC40350.20268427315711773No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40070.20127778997288No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39960.20072524300764372No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38860.19519977335528116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38050.19113101842945054No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37460.18816735743409246No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC37040.18605763265773584No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC37010.1859069380308532No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT36710.18439999176202707No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGAATTTTACTCATCTCTGGG36130.18148656230896318No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT36070.18118517305519793No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35340.17751827046772095No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34540.17349974708418453No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34220.17189233773076998No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33720.16938076061605972No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33250.16701987812823207No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGC32530.1634032070830493No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32000.16074093534145645No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG31190.15667218041562583No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC30970.15556708648515333No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC30740.1544117610123866No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC30540.15340713016650248No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA29780.1495895329521429No Hit
CTATTACTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT29670.14903698598690665No Hit
CTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCC29600.1486853651908472No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA29380.1475802712603747No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28710.14421475792666297No Hit
GTGTATTACTGTGCGAGAGAGGCAGTGACTGGCCCAGATTACTGGGGCCA28560.14346128479224987No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28490.14310966399619043No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27720.13924183523953665No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATCTGG27290.13708187892088583No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26630.13376659712946828No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC26400.13261127165670158No Hit
CCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCA26160.13140571464164064No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT25790.12954714757675503No Hit
GTATATCCTGTAGGTAGTAATAGTTATGCCACTGCGCAAGGTGCAGGTGA25790.12954714757675503No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG25350.12733695971581002No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25300.127085802004339No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC25290.12703557046204478No Hit
CCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCAGA24860.12487561414339399No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG24780.12447376180504033No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT24600.12356959404374464No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTG24040.12075662767526915No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23970.12040500687920971No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATCTGGCACAGCAACTCTT23960.12035477533691552No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGG23890.12000315454085608No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23210.11658740966485012No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA22750.1142767587193167No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG21960.11030846687807448No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG21810.10955499374366141No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21610.10855036289777731No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21500.10799781593254104No Hit
GTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGC21400.10749550050959898No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20930.10513461802177135No Hit
GGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT20790.10443137642965247No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC20780.10438114488735828No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA20650.10372813483753361No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20630.1036276717529452No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT20590.10342674558376838No Hit
GGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGGTAGTA20470.10282396707623792No Hit
ACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGC20320.10207049394182484No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20240.10166864160347121No Hit
ATCCTGTAGGTAGTAATAGTTATGCCACTGCGCAAGGTGCAGGTGAGACT20200.10146771543429439No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA20120.10106586309594073No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG20040.1006640107575871No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19960.10026215841923344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)