FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252013
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60490.48314194820660805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54630.43633732237604556No Hit
CTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTG51550.4117369388337022No Hit
GTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGGTAATG48670.38873398279410837No Hit
GCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT48440.38689694116594636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45560.3638939851263525No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41160.32875058006586194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39950.319086143674227No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39130.3125366909129538No Hit
TGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCAT37830.30215341214508157No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34450.27515688734861377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32250.2575851848183685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31760.25367148743663204No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAA31360.2504766324311329No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30820.24616357817370907No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30510.24368756554444723No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27880.22268139388329036No Hit
GATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCAGCAGCTTAGTAGTTAC27490.21956641025292867No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27260.21772936862476666No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27120.21661116937284197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26530.21189875823973076No Hit
GTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGA25870.20662724748065714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25740.2055889196038699No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25420.2030330355994706No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25340.2023940645983708No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG25180.20111612259617112No Hit
GCTTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA25080.20031740884479632No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24510.19576474046196007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24380.19472641258517284No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24180.19312898508242327No Hit
GGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCATA22500.17971059405932688No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC22200.1773144528052025No Hit
GTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG21240.16964680079200456No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21040.16804937328925498No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT20750.16573310341026812No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20650.16493438965889332No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT20500.16373631903183114No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20100.160541464026332No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20050.16014210715064459No Hit
GATCTATGATGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA19850.15854467964789504No Hit
ATGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA19800.15814532277220764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19720.1575063517711078No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19310.1542316253904712No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19150.15295368338827153No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18980.15159587001093439No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18660.1490399860065351No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG17940.14328924699663662No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17900.1429697614960867No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17720.14153207674361207No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17640.14089310574251226No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17620.1407333629922373No Hit
CAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG17570.1403340061165499No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17540.14009439199113746No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17500.13977490649058755No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17380.1388164499889378No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGG17280.13801773623756303No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17000.13578133773371362No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCGTCCTCC16940.13530210948288876No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16700.13338519647958927No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16600.1325864827282145No Hit
TCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC16470.13154815485142726No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16470.13154815485142726No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16130.128832528096753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16070.1283532998459281No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15970.12755458609455333No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15890.1269156150934535No Hit
GGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAAT15880.12683574371831602No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15870.12675587234317853No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15410.12308178908685453No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15370.12276230358630462No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTG15340.12252268946089218No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15180.12124474745869253No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15170.12116487608355504No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15050.12020641958190528No Hit
CAGCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTT15000.11980706270621792No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG14940.11932783445539304No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14690.11733105007695607No Hit
TTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGA14600.11661220770071877No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14450.11541413707365658No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14310.11429593782173188No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14140.11293812444439476No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13970.11158031106705761No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA13880.11086146869082031No Hit
AGTCAGGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAA13640.10894455568752082No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA13540.10814584193614603No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT13510.1079062278107336No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13320.10638867168312151No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13300.10622892893284654No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13250.10582957205715915No Hit
GACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13170.10519060105605932No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13110.10471137280523445No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13070.10439188730468453No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12980.10367304492844723No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12940.10335355942789731No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12830.10247497430138505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT150.002485999868.06869513
CGCGTGT200.00737797551.71659539
GTCGAAT200.007452609551.5851137
GTCGACG200.007709110651.14548527
GTACGGC200.007764130451.05356214
CGTCTAG200.007766582551.04948410
TCGTATG1900.045.422440
ATCGTAT405.0213406E-543.09716439
CGTATGC1850.042.98245241
GGCGAAT3050.041.5013832
AATCTCG1800.040.08131436
GTATCAA37850.039.933611
ATGCCGT2000.039.90122244
GCGAATC2250.039.58468633
TAAGGCG3300.039.30723629
CGAGACT3300.039.21925723
CGAATGT350.001390761339.1780434
CTTATAC3400.039.0487561
ACGAGAC3500.038.91815622
TATACAC3500.038.905723
Produced by FastQC (version 0.11.5)