FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252013
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56330.44991545614941697No Hit
CTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTG49550.39576266380620645No Hit
GTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGGTAATG47420.37875006090192354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45130.36045951599544096No Hit
GCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT42340.33817540233208443No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41170.3288304514409994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41140.32859083731558697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36530.2917701333772093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34410.2748374018480639No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32680.26101965394928006No Hit
TGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCAT31640.2527130309349823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30800.2460038354234341No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29860.2384959261605111No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29220.23338415815171248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28730.22947046076997601No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27990.2235599790098026No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27650.22084435225512836No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAA26380.21070068761266858No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26360.2105409448623936No Hit
GATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCAGCAGCTTAGTAGTTAC25470.203432392475158No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25120.20063689434534626No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG24320.19424718433434796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24050.19209065720563603No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23900.19089258657857386No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23580.18833670257417454No Hit
GTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGA22750.18170737843776383No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22580.18034956506042668No Hit
GCTTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA22430.1791514944333645No Hit
GTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG22360.17859239480740216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22140.1768352245543776No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21700.17332088404832857No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21040.16804937328925498No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC20490.16365644765669365No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20430.1631772194058688No Hit
GGGCATTACCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCATA20280.1619791487788066No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19930.15918365064899487No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19920.15910377927385738No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19860.1586245510230325No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19700.15734660902083286No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19570.15630828114404563No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19530.15598879564349571No Hit
ATGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA18690.14927960013194752No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT18420.1471230730032356No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18140.1448866744993862No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18040.14408796074801142No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17720.14153207674361207No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17650.14097297711764975No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGG17480.1396151637403126No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17210.13745863661160068No Hit
GATCTATGATGCATACACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA17130.13681966561050085No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT17110.13665992286022588No Hit
CAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTGCCAGATCCACTGCCG17100.1365800514850884No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16640.1329059682287644No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG16600.1325864827282145No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16310.1302702128492276No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16190.12931175634757786No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16180.1292318849724404No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15720.12555780171611636No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15710.1254779303409789No Hit
GGTAACTACTAAGCTGCTGACAGTAATAAGCTGCAAAATCTTCAGGCTGCAGGCTGCTGATGCTGAGAGTGAAAT15630.12483895933987905No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15560.12427985971391671No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15540.12412011696364175No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15500.12380063146309184No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15160.12108500470841756No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15150.12100513333328008No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14760.11789014970291843No Hit
TCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC14730.11765053557750597No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCGTCCTCC14730.11765053557750597No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14640.11693169320126867No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14300.1142160664465944No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14180.11325760994494466No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13850.11062185456540788No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13710.10950365531348318No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13630.10886468431238333No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13480.10766661368532116No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13340.10654841443339645No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13340.10654841443339645No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13240.10574970068202168No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCAGCATCAGCAGCCTGCAGCCTGAAGATTTTG13170.10519060105605932No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12920.10319381667762235No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12910.10311394530248487No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG12780.10207561742569765No Hit
TTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTTCGGCGGAGGGA12750.10183600330028522No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12580.10047818992294807No Hit
CAGCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCAGCAGCTTAGTAGTTACCCTCTCACTTT12540.10015870442239816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)