FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398716
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCACGGGGAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA63460.4537018236725683No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45440.32486938020298617No Hit
GTATGCAGACTCTGTGAAGGGCCGATTCATCATTACCAGAGACAACGGCAAAAACTCCCTGTCTCTGCTGATGAA42650.30492251464914966No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA41740.2984165477480775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41000.29312598125709577No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32590.23299940802850613No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30650.21912954452512162No Hit
GTGTATTACTGTCAGCAGTATGGAAGCTCACGGGGAGTCACTTTCGGCCC30120.21534035501131038No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29270.2092633529608584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29060.2077619759836879No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29030.20754749355837784No Hit
GTATGGAAGCTCACGGGGAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT27050.1933916534879132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25410.18166661423762936No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATT25250.180522707969309No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24720.17673351845549776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24420.17458869420239706No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24020.17172892853159613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23420.1674392800253947No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23210.16593790304822423No Hit
GTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCAGTGGCCCTCTT22770.1627921608103432No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22310.15950343028892214No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC22020.15743010017759143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21490.15364091066378022No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21140.1511386157018294No Hit
GTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20940.1497087328664289No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20490.14649149648677787No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20250.14477563708429733No Hit
GTCTGCATACTGTGTGGTAGAACCATCCCAACTAATAAGAGAGACCCACT20100.14370322495774696No Hit
CTTCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTC19980.14284529525650666No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19410.13877012917561535No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19230.13748323462375495No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18820.13455197481118397No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18080.12926140832020225No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17600.12582968951524112No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGC17530.12532923052285097No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17380.12425681839630062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17260.12339888869506033No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17070.12204050000142988No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16760.11982418160655917No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16760.11982418160655917No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACTCCCCGTGAGCTTCCATACTG16620.11882326362177884No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC16470.11775085149522849No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16470.11775085149522849No Hit
GACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGAAGCTCA16110.11517706239150764No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15930.11389016783964721No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15900.11367568541433715No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15600.11153086116123645No Hit
TCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGACAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCACT15280.1092430486245957No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15260.10910006034105565No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15000.10724121265503506No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14990.10716971851326502No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAT14840.10609730638671468No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14690.10502489426016431No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14400.10295156414883364No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14280.10209363444759337No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14210.1015931754552032No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14120.10094972817927299No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13990.10002030433626269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)