FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398716
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCACGGGGAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA58300.4168108465192362No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43450.3106420459907515No Hit
GTATGCAGACTCTGTGAAGGGCCGATTCATCATTACCAGAGACAACGGCAAAAACTCCCTGTCTCTGCTGATGAA40530.2897657565939047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39400.28168691857389205No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA38730.2768968110753005No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31370.22427712273256328No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31320.22391965202371317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30920.22105988635291227No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29900.21376748389236988No Hit
GTGTATTACTGTCAGCAGTATGGAAGCTCACGGGGAGTCACTTTCGGCCC28440.20332933919394647No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26050.18624223931091088No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24460.17487467076947716No Hit
GTATGGAAGCTCACGGGGAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT24310.1738022586429268No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATT24140.1725868582328364No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23550.16836870386840502No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23270.16636686789884436No Hit
GTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCAGTGGCCCTCTT22120.15814504159529166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21620.1545703345067905No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21230.1517820629777596No Hit
GTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20380.14570506092730762No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19970.14277380111473667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19850.14191587141349638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19740.14112943585402613No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19710.14091495342871604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19630.14034300029455588No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19480.1392705881680055No Hit
GTCTGCATACTGTGTGGTAGAACCATCCCAACTAATAAGAGAGACCCACT19440.13898461160092543No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC19270.13776921119083502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18640.13326508025932354No Hit
CTTCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTC18340.13112025600622287No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17860.12768853720126172No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17320.12382785354568047No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17130.12246946485205003No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16980.12139705272549968No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACTCCCCGTGAGCTTCCATACTG16970.12132555858372965No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16970.12132555858372965No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16940.12111107615841957No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGC16330.11674993351044816No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC15440.11038695489291607No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14790.10573983567786456No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14670.10488190597662428No Hit
GACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGAAGCTCA14510.10373799970830389No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14470.1034520231412238No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAT14350.10259409343998352No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14340.10252259929821349No Hit
GTAATAGAAGGCGGTGTCCTCAGGTCTCAGTCTGTTCATCAGCAGAGACAGGGAGTTTTTGCCGTTGTCTCTGGT14190.10145018717166315No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14150.10116421060458307No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14120.10094972817927299No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14110.10087823403750297No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14090.10073524575396292No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14040.1003777750451128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.039.7493061
CGCCTAT350.001394408339.15749432
GTATCAA39950.037.3340151
TATTAGG852.4556357E-937.2463556
TATCAAC44000.033.5013472
ATCAACG45750.032.0466163
TCAACGC46400.031.512934
CAACGCA47050.031.2213445
GACAGGT3500.031.204841
AACGCAG47450.031.1018076
CGCAGAG49450.029.8438998
GAGTTTA6200.029.7263851
ACGCAGA49700.029.6937777
AGAGTAC50350.029.23856511
GCAGAGT51300.028.7676549
AATAGTC1202.8176146E-928.62230736
CGGGTAC2750.028.52388817
ATATAGC605.822962E-428.4022456
AATGGTA6600.028.29596170
GTGGTGA5850.028.2401570
Produced by FastQC (version 0.11.5)