FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760570
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG42460.2411718931936816No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34580.19641366148463285No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACAAGCAGTACTACTATCCCACAC34360.19516406618311116No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32460.18437210676087856No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTGCACTGGTCCCAGCAGAGGCCAGGCCAGGCCC30700.17437534434870525No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30250.17181935395922912No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27820.158017005856058No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACAAGCAGTA27650.15705140948670032No Hit
CAGTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG26730.15182582913488246No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24850.14114746928551547No Hit
GTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC24720.14040907206188905No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGGATCCCT22520.12791311904667238No Hit
GTAGTACTGCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA22070.12535712865719625No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT21850.12410753335567459No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC21200.12041554723754239No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGGACCAGTGCACACTTTCACTT20890.11865475385812549No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20790.11808675599379746No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCC20710.11763235770233504No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19880.11291797542841239No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC19650.11161158034045791No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19650.11161158034045791No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19490.11070278375753308No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19360.10996438653390662No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18970.10774919486302731No Hit
TAGTAGTACTGCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC18740.10644279977507286No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18700.10621560062934164No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18520.10519320447355117No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18340.10417080831776072No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT18320.10405720874489512No Hit
GGATAGTAGTACTGCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18280.10383000959916391No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA17850.10138761878255337No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC17700.10053562198606132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47200.037.305161
GGTATCA19250.035.884511
CGTATGC3650.034.0674141
TCGTATG3350.033.9502740
TATCAAC53000.033.093052
ACGTAGA5450.032.3616427
GACGTAG5450.032.34507426
ATCAACG54850.031.9151463
AATCTCG3550.031.81224336
ATCTCGT3450.031.78710237
TCAACGC55400.031.537184
CGTAGAG5600.030.90936728
AGGGGGG5950.030.88419270
CAACGCA56750.030.8466195
TCTCGTA3250.030.63705338
CGAGACG5750.030.6322423
AACGCAG57800.030.4619986
AGACGTA5900.029.86533525
ACGAGAC6350.028.8030622
TGGTATC7550.028.2537772
Produced by FastQC (version 0.11.5)