FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452115
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT57380.395147767222293No Hit
CTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA40310.2775950940524683No Hit
CTACTACACTGCTCACAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC36700.25273480406166177No Hit
GTATTACTGTGAGCAGTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGA33790.23269506891671804No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32700.22518877637101742No Hit
GTATTATTGTACAAGTGCCCCTCGCACATTCATTGCGCCTCCCTGGGACTACTGGGGCCAGGGAACCCTGGTCAC31520.21706269820227736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31120.21430809543321294No Hit
CAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCA30080.20714612823364542No Hit
GGCCAATAGTGTACGGCGAGCTACTACACTGCTCACAGTAATACATTCCA24030.16548276135154585No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22700.1563237071444066No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22640.15591051672904693No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21560.14847308925257297No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTCTTT21340.14695805772958753No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21190.14592508169118837No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20950.1442723200297497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20250.13945176518388694No Hit
CAATAATACAGGGCCGTGTCTTCGGGTCTCAGGCTGTTCATTTGCAGATA20190.1390385747685273No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20080.13828105900703455No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19620.13511326582261046No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19110.13160114729205333No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19000.13084363153056058No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18790.12939746507680178No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18320.12616080682315106No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATCAAGTCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG18110.12471464036939223No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17740.12216663280800763No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17620.1213402519772883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17510.12058273621579557No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16510.1136962292931345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16440.11321417380854822No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15800.10880680937804513No Hit
GTCCCCAGGCACCCTGTCATTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGCCAA15780.1086690792395919No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15660.10784269840887259No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15640.10770496827041937No Hit
CTTTATATCTGCAAATGAACAGCCTGAGACCCGAAGACACGGCCCTGTAT15160.10439944494754204No Hit
CCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATCAAGTCATGA15050.10364192918604932No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14960.10302214356300982No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14840.10219576273229047No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14820.10205803259383728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)