FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452115
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT50100.3450139968253203No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36440.2509443122617699No Hit
CTACTACACTGCTCACAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC35640.24543510672364105No Hit
CTATTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA35210.24247390874689678No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33310.2293895455938407No Hit
GTATTATTGTACAAGTGCCCCTCGCACATTCATTGCGCCTCCCTGGGACTACTGGGGCCAGGGAACCCTGGTCAC30150.20762818371823166No Hit
GTATTACTGTGAGCAGTGTAGTAGCTCGCCGTACACTATTGGCCAGGGGA29710.2045981206722608No Hit
CAGTAATACATTCCAAGATCTCCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTCCCA28200.19419949521904256No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24670.16989012578204893No Hit
GGCCAATAGTGTACGGCGAGCTACTACACTGCTCACAGTAATACATTCCA22450.15460208041374132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21880.15067677146782452No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21090.14523643099892228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20940.1442034549605231No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTCTTT20030.13793673366090153No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAATCAAGTCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG19070.13132568701514688No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18380.12657399723851073No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18280.12588534654624461No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18130.12485237050784545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18070.1244391800924858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18000.12395712460789951No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16640.11459147519308044No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16620.11445374505462722No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16330.11245665804705551No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16180.11142368200865634No Hit
CAATAATACAGGGCCGTGTCTTCGGGTCTCAGGCTGTTCATTTGCAGATA16020.11032184090103056No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16000.11018411076257734No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15760.10853134910113868No Hit
CTTTATATCTGCAAATGAACAGCCTGAGACCCGAAGACACGGCCCTGTAT15110.10405511960140898No Hit
GTCCCCAGGCACCCTGTCATTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGCCAA14620.10068073120930504No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14560.10026754079394538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.043.497291
TGGTATC6350.041.2320442
GGTATCA17750.039.287371
GTATCAA41750.036.583671
TATCAAC46700.032.182762
ATCAACG48250.031.3538463
TCAACGC48550.030.945774
CAACGCA48750.030.817755
AACGCAG49700.030.3654636
CATCTGA20000.030.29421270
ACGTCCG450.004972278630.2134827
ACGCAGA53200.028.4316277
CGCAGAG53100.028.4211588
GCAGAGT54550.027.601489
AGAGTAC54750.027.49970411
CAGAGTA58250.025.67320810
GCTTGTA1751.0913936E-1125.2698541
CAGTTCG12700.024.3958470
TCTCGCG8500.024.30016970
AGAGTCG700.001455755724.27618411
Produced by FastQC (version 0.11.5)