FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807051
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71540.3958936410759851No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67520.3736474510127274No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60870.33684716148022387No Hit
GCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAAACCGTCGCCATCTGTATATGAGCAG56380.31200004869812753No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA53300.2949557040725469No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51350.28416464172842937No Hit
CTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC50540.2796822004470267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT50170.2776346655407069No Hit
GTATATGAGCAGCAATAGTAATCAGCCTCGTCCTCAGTCTGGAGCCCAGA50160.27757932675945507No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTC49210.27232214254052595No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT48970.27099401179048077No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG48320.2673969910091082No Hit
CATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCC47680.26385530900898757No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCGATCACCATCTCCTG46820.25909617382132544No Hit
CTCCTGCACTGGAGACAGCAGTAATGTTGGGACTTATAATTATGTCTCCTGGTATCAACAATATTCAGGCAAGGC45180.25002061369601636No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGACAGCAGTAATGTT44120.2441547028833165No Hit
GTCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC43920.2430479272582788No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG43040.23817811450811294No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC41710.23081805660161223No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41090.22738705216399538No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG41050.2271656970389878No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCGATCAC40600.224675451882653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40330.22318130478885212No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA40270.2228492721013408No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39190.21687268372613724No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG39110.21642997347612217No Hit
GGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT37750.20890389922586577No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC36960.20453213550696686No Hit
GTCGATCACCATCTCCTGCACTGGAGACAGCAGTAATGTTGGGACTTATA36810.2037020537881886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36710.20314866597566975No Hit
GACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCGATCACCATCT35910.19872156347551895No Hit
GATTACTATTGCTGCTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGT35750.1978361429754888No Hit
CTGTATATGAGCAGCAATAGTAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGC35390.19584394685042092No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC35330.19551191416290964No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC34950.193409040475338No Hit
GCTCCAGACTGAGGACGAGGCTGATTACTATTGCTGCTCATATACAGATG34200.18925863188144662No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32930.18223060666245722No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA32220.17830155319357338No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC31300.1732103853184No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACAC30980.17143954431833966No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29510.16330474347431256No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT29150.16131254734924472No Hit
CCTTGGTCCCTCCGCCGAACACCAAACCGTCGCCATCTGTATATGAGCAG29130.16120186978674095No Hit
ATAGTAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATTGTCAGGGAGG28340.15683010606784203No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC27980.1548379099427742No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT27360.15140690550515729No Hit
GACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAAACCGTCGCCATCTGTATATGAGCAGCAATAGTAAT27010.14947004816134132No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG26200.14498760687993864No Hit
GCTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTG25830.1429400719736189No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25380.14044982681728407No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG25320.14011779412977277No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25120.13901101850473507No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25090.13884500216097942No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24700.1366867896921559No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC24670.13652077334840024No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC24230.13408586697331729No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23900.1322596871920051No Hit
TCTCTGGGCTCCAGACTGAGGACGAGGCTGATTACTATTGCTGCTCATAT23720.13126358912947117No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23330.12910537666064764No Hit
GCACTGGAGACAGCAGTAATGTTGGGACTTATAATTATGTCTCCTGGTAT23310.12899469909814387No Hit
GTTTGGGGGCCTTGCCTGAATATTGTTGATACCAGGAGACATAATTATAA23270.12877334397313633No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTACTGC23230.1285519888481288No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23180.12827529494186937No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAAACCGTCGC22980.12716851931683168No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC22950.12700250297307603No Hit
CCCATGAGGGCCGCTGACTGACCTCATACATGAGAAGTTTGGGGGCCTTGCCTGAATATTGTTGATACCAGGAGA22940.12694716419182414No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGATGCAGACATGGAGGCAGG22920.12683648662932037No Hit
CTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGC22910.1267811478480685No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC22770.12600640491054207No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22130.12246472291042144No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA22080.12218802900416204No Hit
GTATGAGGTCAGTCAGCGGCCCTCATGGGTTTCTAGTCGTTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC21830.12080455947286489No Hit
ACAATATTCAGGCAAGGCCCCCAAACTTCTCATGTATGAGGTCAGTCAGCGGCCCTCATGGGTTTCTAGTCGTTT21800.12063854312910927No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG21660.11986380019158285No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC21410.11848033066028574No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC21220.11742889381649992No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC21160.11709686112898862No Hit
GGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG21150.11704152234773672No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG21140.11698618356648484No Hit
ACCATGGCCTGGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT21120.11687550600398107No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC21020.11632211819146221No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT20980.11610076306645467No Hit
GCAGTAATGTTGGGACTTATAATTATGTCTCCTGGTATCAACAATATTCA20930.11582406916019526No Hit
GTCCAGGAGATGCAGACATGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC20830.11527068134767642No Hit
CCCTGACAATCTCTGGGCTCCAGACTGAGGACGAGGCTGATTACTATTGC20270.11217170959757085No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA20260.11211637081631896No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCG20230.11195035447256331No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC20140.11145230544129633No Hit
CATTACTGCTGTCTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAT20000.11067756250376995No Hit
GGCCTGGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19820.10968146444123603No Hit
TATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC19680.10890672150370964No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA19610.10851935003494645No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19120.10580774975360407No Hit
CTATTGCTGCTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCC19050.10542037828484088No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT18690.10342818215977301No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG18660.10326216581601738No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT18510.10243208409723908No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18370.1016573411597127No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG18310.10132530847220139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT200.007477395251.5427537
AATATCG200.00750547651.4939136
GTGGTAT4100.048.2286571
AGGGGGG3950.048.0329570
CGCGTAT306.358317E-445.9323939
GCGTATG503.847368E-641.39472240
CGTATGC3250.039.32478341
GATTAAC350.001429058638.963051
AGACGTA5000.038.19330625
GTCACCG4850.037.67074270
AATCGCG557.652785E-637.4501236
CGAGACG5300.037.30483223
CCCACGA5350.036.95107319
ATGCCGT3100.036.9196544
GACGTAG5250.036.38162626
TCGTATG3050.036.1921140
CGTAGAG5200.036.09752728
TCTCCGA5500.035.9393411
TATACAC5850.034.964913
ACGTAGA5400.034.7461427
Produced by FastQC (version 0.11.5)