FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807051
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC68290.37790853716912254No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67330.37259601416894156No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56810.3143796162919586No Hit
GCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAAACCGTCGCCATCTGTATATGAGCAG52300.28942182594735844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG48500.2683930890716421No Hit
GTATATGAGCAGCAATAGTAATCAGCCTCGTCCTCAGTCTGGAGCCCAGA47170.2610330311651414No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT46240.25588652450871613No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA45690.25284289153986245No Hit
CTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC45160.24990993613351256No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43910.24299258847702696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42800.2368499837580677No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42500.23518982032051114No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTC42430.23480244885174797No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCGATCACCATCTCCTG42410.23469177128924418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42030.23258889760167256No Hit
CTCCTGCACTGGAGACAGCAGTAATGTTGGGACTTATAATTATGTCTCCTGGTATCAACAATATTCAGGCAAGGC41950.23214618735165748No Hit
CATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCC41560.22998797488283396No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40950.226612309226469No Hit
GTCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC40770.22561621116393504No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG40000.2213551250075399No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCGATCAC38430.21266693635099396No Hit
GGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT37290.2063583152882791No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36680.2029826496319141No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG36360.20121180863185376No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36110.19982833910055664No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACAC36020.19933029006928968No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35990.19916427372553402No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35530.19661868978794733No Hit
GTCGATCACCATCTCCTGCACTGGAGACAGCAGTAATGTTGGGACTTATA35190.19473717122538323No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34720.19213624850654465No Hit
GATTACTATTGCTGCTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGT34490.19086345653775127No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC32930.18223060666245722No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGACAGCAGTAATGTT32700.18095781469366387No Hit
CTGTATATGAGCAGCAATAGTAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGC32670.18079179834990822No Hit
GCTCCAGACTGAGGACGAGGCTGATTACTATTGCTGCTCATATACAGATG32370.17913163491235168No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32360.1790762961310998No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32110.17769282659980268No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31190.17260165872462926No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31160.17243564238087358No Hit
GACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCGATCACCATCT30480.16867260525574543No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC28780.15926501244292496No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27370.15146224428640917No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT27200.15052148500512713No Hit
CCTTGGTCCCTCCGCCGAACACCAAACCGTCGCCATCTGTATATGAGCAG26800.14830793375505175No Hit
ATAGTAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATTGTCAGGGAGG26520.14675844787999895No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25820.14288473319236702No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24090.1333111240357909No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23840.13192765450449379No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC23530.13021215228568536No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT23500.1300461359419297No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC23270.12877334397313633No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23190.12833063372312126No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23040.127500552004343No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG22930.12689182541057226No Hit
GACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAAACCGTCGCCATCTGTATATGAGCAGCAATAGTAAT22900.1267258090668166No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22750.12589572734803833No Hit
GTTTGGGGGCCTTGCCTGAATATTGTTGATACCAGGAGACATAATTATAA22690.12556369466052703No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC22660.12539767831677137No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA22340.12362683731671105No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGACAGCAGTAATGTTGGGACTTATAATTATGTCTCCTGGT22240.1230734495041922No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAAACCGTCGC21960.1215239636291394No Hit
GCTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTG21830.12080455947286489No Hit
TCTCTGGGCTCCAGACTGAGGACGAGGCTGATTACTATTGCTGCTCATAT21480.11886770212904893No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21230.11748423259775181No Hit
CTCATATACAGATGGCGACGGTTTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGC21010.11626677941021034No Hit
CCCATGAGGGCCGCTGACTGACCTCATACATGAGAAGTTTGGGGGCCTTGCCTGAATATTGTTGATACCAGGAGA20880.11554737525393582No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC20790.11504932622266888No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG20440.11311246887885289No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCATCTCCTGGACAGTCG20370.1127250974100897No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20330.11250374228508216No Hit
GGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG20260.11211637081631896No Hit
GGCCTGGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20260.11211637081631896No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT20160.11156298300380012No Hit
GCACTGGAGACAGCAGTAATGTTGGGACTTATAATTATGTCTCCTGGTAT19980.1105668849412662No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC19950.11040086859751054No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGATGCAGACATGGAGGCAGG19940.11034552981625864No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA19910.110179513472503No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG19670.10885138272245774No Hit
GTCCAGGAGATGCAGACATGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC19420.10746791319116063No Hit
ACAATATTCAGGCAAGGCCCCCAAACTTCTCATGTATGAGGTCAGTCAGCGGCCCTCATGGGTTTCTAGTCGTTT19220.10636113756612292No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19130.10586308853485595No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19110.10575241097235219No Hit
GTATGAGGTCAGTCAGCGGCCCTCATGGGTTTCTAGTCGTTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC18930.10475631290981827No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTACTGC18810.10409224753479564No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18790.10398156997229187No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18780.10392623119103998No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18630.10309614947226171No Hit
ACCATGGCCTGGGCTCTACTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT18580.10281945556600228No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGT18470.10221072897223156No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG18390.10176801872221648No Hit
CATTACTGCTGTCTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAT18160.10049522675342312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)