FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195102
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC58580.4901673664674647No Hit
GTATAATAGTTATTCTCAAATGTACACTTTTGGCCAGGGGACCAAGCTGG49910.41762125743241996No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49450.4137722135851166No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47320.3959494670747769No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTC40470.33863218369645437No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36800.3079235077842728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36400.3045765131344438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34780.29102118480263606No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34250.28658641689161257No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29190.2442469345712751No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28840.24131831425267466No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA28390.237552945271617No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27180.22742828645588412No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26960.2255874393984781No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26930.22533641479974093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26570.22232411961489482No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24210.20257685118090338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23540.19697063514243973No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22820.19094604477274743No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG22790.19069502017401027No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTA22590.18902152284909574No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA22120.1850888041355466No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA22020.18425205547308932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21060.1762192683134996No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21040.17605191858100813No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20290.16977630361257867No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20040.16768443195643554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19240.1609904426567774No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19130.16007001912807442No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18830.15755977314070263No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18700.1564719998795082No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18650.15605362554827956No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18620.15580260094954237No Hit
TCATAGATCAGGAGCTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGC18320.15329235496217058No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17970.15036373464357017No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA17750.1485228875861642No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17740.14843921271991847No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17710.14818818812118129No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17090.14300034641394627No Hit
CAGTATAATAGTTATTCTCAAATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA17000.1422472726177347No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16390.13714310577674543No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATTTGAGAATAACTATTATA16090.13463285978937362No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16060.13438183519063643No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15980.13371243626067064No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15940.13337773679568773No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15930.13329406192944201No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15890.1329593624644591No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15840.13254098813323048No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15660.13103484054080738No Hit
ATTATACTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGC15220.12735314642599543No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15180.12701844696101253No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTAGTTTCTG15110.12643272289729246No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15080.12618169829855527No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15010.12559597423483518No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14780.12367145231118347No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14760.12350410257869202No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14550.12174693038753179No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14500.12132855605630315No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14430.12074283199258307No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14390.12040813252760016No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14340.11998975819637152No Hit
GGCCAAAAGTGTACATTTGAGAATAACTATTATACTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGG14280.11948770899889716No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14000.11714481274401684No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA13800.1154713154191023No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13620.11396516782667923No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13540.11329576889671342No Hit
GTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCT13490.11287739456548479No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13180.11028347371186728No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13130.10986509938063864No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12960.10844262665446129No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12700.10626708013207241No Hit
GCTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACCAGTTACTA12370.10350580954596345No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12050.10082821382610019No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCTAA12030.10066086409360873No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12030.10066086409360873No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12020.10057718922736301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)