FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844468
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30410.36010837592425055No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28590.3385563455335193No Hit
GAGTTACAGTTACCCACGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT24710.29261025876646596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21850.2587427824381741No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC21240.25151929972479714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20610.24405898151262095No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA20390.24145379102582928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19820.23470397931005085No Hit
GGCCAAAACTGCGTGGGTAACTGTAACTCTGTTGACAGTAGTAAGTTGCA19040.2254673948568803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18200.21552030390731206No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17530.2075863146975374No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16560.19609979300577404No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16150.19124466528038955No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16090.1905341587839918No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15750.18650795530440467No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT14960.17715295310183454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14380.17028472363665645No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGATCAGTGGCAGTGGATCTGGGACAGATTTCA13910.16471908941487423No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13880.16436383616667535No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13780.16317965867267914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13750.16282440542448026No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13080.1548904162147056No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11980.14186446378074716No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11940.14139079278314867No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11920.14115395728434943No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG11860.1404434507879517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGCGTGGGTAA11860.1404434507879517No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11830.14008819753975285No Hit
ACTTACTACTGTCAACAGAGTTACAGTTACCCACGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAA11650.13795667805055964No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11590.1372461715541619No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11450.1355883230625672No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11350.134404145568571No Hit
CCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGGAGATACCAATT11130.1317989550817793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11130.1317989550817793No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11070.13108844858538157No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTAC11050.13085161308658233No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10860.1286016758479895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10680.1264701563587963No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10220.1210229398864137No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10120.11983876239241746No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9930.11758882515382466No Hit
GTCTGCATCTGTAGGTGACAGAGTCACCTTCACTTGTCGGGCAAGTCAGA9830.11640464765982844No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9820.11628622991042882No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9630.114036292671836No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC9420.11154951993444394No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGTGACAGAGTCACCTTCACTTGTCGGGCAAGTCAGACCATTAGTAC9290.11001008919224885No Hit
GTAGGTGACAGAGTCACCTTCACTTGTCGGGCAAGTCAGACCATTAGTACCTATTTAAATTGGTATCTCCAGAAA9170.10858907619945338No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8980.10633913896086057No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8980.10633913896086057No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8930.10574705021386245No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8800.10420761947166736No Hit
CCATTAGTACCTATTTAAATTGGTATCTCCAGAAACCAGGGAAAGCCCCT8780.10397078397286813No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8750.10361553072466927No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC8670.1026681887294723No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGATCCTTGATGGGACCCCA8590.10172084673427531No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8530.10101034023787757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG150.00258865867.375579
GTGGTAT2150.053.283171
TTTGGCG200.00806048450.57060222
GGTATCA8650.040.8999521
ACCGTAC3250.039.52218227
GTATCAA24050.039.0875741
GACCGTA3300.038.89101426
TCGTATG3100.038.86063440
CGTATGC3200.038.79930541
CGAATAA350.001510609438.52313621
TACTAGA3400.037.9431131
ATGCCGT3300.036.8015544
CGTACTA3550.036.251229
CTAGATC2550.036.047233
GTACTAG3600.035.7796530
TATCAAC26600.035.5937922
ATCTCGT2550.034.91897637
ATCGTAT400.00264303534.3736739
ATATCGT400.002691144834.24745637
TCAACGC27500.034.183864
Produced by FastQC (version 0.11.5)